Breeding dominant genic male sterility restorer line of Brassica napus L.  

作　　者：Junying Zhang Jianxia Jiang Liyong Yang Meiyan Jiang Yanli Li Weirong Wang Chaocai Sun Jifeng Zhu Xirong Zhou 

机构地区：[1]Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,China

出　　处：《Oil Crop Science》2020年第2期90-95,共6页中国油料作物学报(英文版）

基　　金：supported by the Youth Talent Development Plan of Shanghai Municipal Agricultural System,China(No.20180103);the National Key Research and Development Program of China(No.2018YFD0100602);the Youth Talent Running Plan of Shanghai Academy of Agricultural Sciences,China(No.2017020)。

摘　　要：To facilate breeding process of Brassica napus,a microspore culture and molecular marker-assisted screening combined system were proposed in this research.At early flowering stage,F1 offspring of hybridized combination HY15 A×HF06 was used as donor for microspore culture to analyze effects of colchicine concentration on embryogenic and diploid rates of microspore.Treatment with 50 mg/L colchicine resulted in embryogenic rate of3.56 embryos/bud,which was substantially higher than control(0.78 embryos/bud).A total of 1,387 embryos and 862 single plants were obtained after induction culture.Ploidy detection was performed for the regenerated plants by flow cytometry.Diploid rates of microspores treated with 50 mg/L and 70 mg/L colchicine were 17.2%and 21.0%respectively,which was significantly higher than control(10.5%).Totally 108 single plants that doubled successfully were randomly selected and screened using molecular marker BE10.Approximately 54 of108 plants generated a 305 bp amplification product,whereas the other 54 plants showed a 398 bp band,thereby satisfying 1:1 separation ratio(x0.052=0.0093).These coincided with field identification results.Findings of this study indicated that homozygous breeding material could be obtained by microspore culture in a short time,thereby remarkably accelerate breeding.

关 键 词：Brassica napus L. MICROSPORES Double haploid Restorer line COLCHICINE 

分 类 号：S565.4[农业科学—作物学]