结构光照明显微镜的实验教学设计  

Experimental Teaching Design of the Structured Illumination Microscopy

作　　者：缴莉 刘如明[1] 龙加福[1] 周颖[1] JIAO Li;LIU Ruming;LONG Jiafu;ZHOU Ying(College of Life Sciences,Nankai University,Tianjin 300071,China)

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《实验室研究与探索》2020年第5期216-219,223,共5页Research and Exploration In Laboratory

基　　金：南开大学基本科研业务费(63140004)。

摘　　要：结构光照明显微成像技术具有超高分辨率、对样品光损伤小、拍摄速度快等优点,被广泛用于细胞内精细结构、蛋白精确定位、活细胞动态观测等科学研究中。在实验教学中加入大型精密仪器结构光照明显微镜DeltaVision OMX SR的内容将增强教学的实用性和应用性。在实验教学设计中重视理论课程教学,通过小组教学、开放性实验等方式培养学生动手和独立思考的能力,培养学生的创新意识,帮助学生提升综合实验能力。Structured illumination microscopy achieves optical super-resolution with a good acquisition speed and low phototoxicity.As one of the most powerful and versatile optical super-resolution techniques,structured illumination microscopy has been used in studying subcellular structure,protein localization and dynamic changes in live cells.Adding large-scale precision instrument structured illumination microscopy DeltaVision OMX SR in experimental teaching would strengthen the practical value of the course.The course design emphasizes the theoretical teaching.Group teaching and open experimental teaching are adopted to improve student ’s operating ability,independent thinking ability,innovation thinking ability and comprehensive experimental ability.

关 键 词：超高分辨显微镜 结构光照明显微镜 实验教学 大型精密仪器 

分 类 号：Q-334[生物学]