T-cadherin基因通过雄激素受体通路对前列腺癌LNCaP细胞增殖的抑制作用  

Inhibition of proliferation of prostate cancer LNCaP cells by T-cadherin gene through androgen receptor pathway

作　　者：王秋菊[1] 宋晓玉[1] 张莉[1] 史敏 陈义波[1] 贺巧[1] 胥萍瑶[1] 倪苏娇 吕梦宇 吴睿 赖琴 赵燕珍 WANG Qiu-ju;SONG Xiao-yu;ZHANG Li;SHI Min;CHEN Yi-bo;HE Qiao;XU Ping-yao;NI Su-jiao;LV Meng-yu;WU Rui;LAI Qin;ZHAO Yan-zhen(Clinical Laboratory of Sichuan Cancer Hospital,Chengdu 610041,Sichuan Province,China;不详)

机构地区：[1]四川省肿瘤医院检验科,四川成都610041 [2]广州医科大学附属第三医院检验科,广东广州510150

出　　处：《中国生物制品学杂志》2020年第6期653-657,共5页Chinese Journal of Biologicals

基　　金：2019年四川省干部保健科研课题-普通项目(2019-802);广州医科大学附属第三医院2018年度青年科研项目(2018Q04)。

摘　　要：目的分析前列腺癌组织中T-cadherin基因mRNA转录水平,探讨T-cadherin基因通过雄激素受体(androgen receptor,AR)通路抑制前列腺癌LNCaP细胞增殖的作用。方法收集前列腺癌及癌旁相应正常组织各60份,实时荧光定量PCR检测组织中T-cadherin mRNA转录水平;将培养24 h的前列腺癌LNCaP细胞分为腺病毒GFP组、腺病毒GFP-T-cadherin组及空白对照组(不含腺病毒),CCK-8法检测T-cadherin对前列腺癌LNCaP细胞增殖的影响,Western blot法检测过表达T-cadherin后前列腺癌LNCaP细胞中AR及前列腺特异性抗原(prostate specific antigen,PSA)蛋白表达水平,并检测不同组别LNCaP胞浆及胞核中AR蛋白表达水平。结果T-cadherin基因在癌组织中较癌旁组织mRNA转录水平明显减少(P<0.01),其转录水平与前列腺癌的分期、格里森评分及分化均有关(P均<0.05);与空白对照组比较,腺病毒GFP-T-cadherin组能抑制前列腺癌LNCaP细胞增殖(P<0.05),而T-cadherin过表达能下调LNCaP细胞中AR及下游靶基因PSA的表达水平(P均<0.05)。结论前列腺癌组织中T-cadherin基因mRNA转录水平明显减少,其过表达能通过AR通路抑制前列腺癌LNCaP细胞的增殖,本研究为前列腺癌的治疗提供了新的靶点。Objective To analyze the T-cadherin mRNA transcription level in prostate cancer and investigate its inhibitory effect on proliferation of prostate cancer LNCaP cells through androgen receptor(AR)pathway.Methods The transcription levels of T-cadherin mRNA in 60 prostate cancer and 60 adjacent normal tissue samples were determined by real-time fluorescent quantitative PCR(qRT-PCR).LNCaP cells were cultured for 24 h and divided into three groups.The cells in GFP and GFP-T-cadherin groups were treated with adenovirus containing GFP,adenovirus containing GFP-Tcadherin respectively,while those in blank control group were untreated.The effect of T-cadherin on proliferation of LNCaP cells was evaluated by CCK-8 assay.After overexpression of T-cadherin,the expression levels of AR and prostate specific antigen(PSA)in LNCaP cells,as well as expression levels of AR in cytoplasm and nuclei,were determined by Western blot.Results The transcription level of T-cadherin mRNA in prostate cancer tissue was significantly lower than that in adjacent normal tissue(P<0.01),which was correlated to the stage,Gleason score and differentiation the cancer(each P<0.05).Compared with that in blank control group,the proliferation level of LNCaP cells in GFP-T-cadherin group decreased significantly(P<0.05).However,the overexpression of T-cadherin down-regulated the expressions of AR and downstream target gene PSA(each P<0.05).Conclusion The transcription level of T-cadherin mRNA in prostate cancer tissue decreased significantly,while its overexpression inhibited the proliferation of LNCaP cells through the AR signaling pathway,which provided a new target for the therapy of prostate cancer.

关 键 词：T-CADHERIN 前列腺癌细胞LNCaP 雄激素受体通路 细胞增殖 

分 类 号：R737.25[医药卫生—肿瘤]