基于mtCOI基因序列的中国葱斑潜蝇不同地理种群遗传多样性分析  

Analysis of the genetic diversity of geographical populations of Liriomyza chinensis(Diptera:Agromyzidae)in China based on mtCOI gene sequence

作　　者：钟裕俊 杜素洁 潘立婷 王玉生[2] 王福莲[1] 刘万学[2] ZHONG Yu-Jun;DU Su-Jie;PAN Li-Ting;WANG Yu-Sheng;WANG Fu-Lian;LIU Wan-Xue(Institute of Entomological Science,College of Agriculture,Yangtze University,Jingzhou,Hubei 434025,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]长江大学农学院,长江大学昆虫研究所,湖北荆州434025 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2020年第6期769-778,共10页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31772236);中国农业科学院科技创新工程项目(caascx-2017-2022-IAS)。

摘　　要：【目的】葱斑潜蝇Liriomyza chinensis是葱蒜类蔬菜上的重要经济害虫,在我国广泛分布。本研究旨在阐析中国葱斑潜蝇的地理种群遗传分化。【方法】以中国8省12个不同地理种群的253头葱斑潜蝇为样本,测定其mtCOI基因序列;依据获得的mtCOI基因序列,利用MEGA7.0,DnaSP 6.1和Arlequin 3.5等软件对葱斑潜蝇地理种群的遗传多样性、基因流水平和遗传变异等进行分析。【结果】在253头个体的759 bp mtCOI基因片段中,获得13个单倍型,各单倍型间K2P遗传距离均小于0.02;其中单倍型Hap1为12个地理种群所共享,总发生频率高达81.82%。总群体单倍型多样性较低(Hd=0.327),核苷酸多样性(Pi)为0.00159,核苷酸平均差异数(K)为1.21011;葱斑潜蝇总群体遗传分化程度中等(F ST=0.06971),基因交流较充分(Nm=3.33629)。分子方差分析(AMOVA)结果表明,遗传变异来源为群体内;总群体Tajima’s D检验值为显著负值;Mantel检测结果说明种群间的遗传距离与地理距离没有相关性。【结论】中国葱斑潜蝇地理种群的遗传多样性较低,基因交流较充分,遗传分化程度中等,且地理距离并不影响其遗传分化程度;葱斑潜蝇总群体在较近的历史时期未经历明显的种群扩张和种群增长。【Aim】Liriomyza chinensis,a serious economic pest of onions and garlics,is widely distributed in China.This study aims to analyze the genetic differentiation among the geographical populations of L.chinensis in China.【Methods】A total of 253 individuals from 12 different geographical populations from 8 provinces were used as samples,and their mtDNA COI gene was sequenced.The genetic diversity,gene flow levels and genetic variation of the geographical populations of L.chinensis were analyzed by MEGA7.0,DnaSP 6.1 and Arlequin 3.5 based on the obtained mtCOI gene sequences.【Results】From the 253 individuals 13 haplotypes of the mtCOI gene fragment of 759 bp in length were defined,and the K2P genetic distance between haplotypes was less than 0.02.Haplotype Hap1 was shared by the 12 geographical populations,and the total occurrence frequency was up to 81.82%.The haplotype diversity(Hd)of all geographical populations was low(0.327),the nucleotide diversity(Pi)was 0.00159,and the average number of nucleotide difference(K)was 1.21011.The total population had generated moderate genetic differentiation(F ST=0.06971),and the gene flow was relatively sufficient(Nm=3.33629).The results of molecular variance analysis(AMOVA)showed that the source of genetic variation was within population,and the Tajima’s D test value was significantly negative.Mantel test results showed that the genetic distance was not related to the geographical distance.【Conclusion】The geographical populations of L.chinensis in China show low genetic diversity,relatively sufficient gene flow and moderate genetic differentiation,and the geographical distance does not affect the degree of genetic differentiation between geographical populations.The total population of L.chinensis has not experienced significant population expansion and population growth in the recent historical period.

关 键 词：葱斑潜蝇 线粒体COI基因 地理种群 遗传多样性 系统进化分析 

分 类 号：Q969[生物学—昆虫学]