解淀粉芽孢杆菌NS2降解玉米赤霉烯酮的研究  

Degradation Effects of Bacillus amyloliquefaciens NS2 on Zearalenone

作　　者：张晨曦 Yawa Minnie Elodie Folly 赵月菊[1] 刘阳[1] ZHANG Chenxi;Yawa Minnie Elodie Folly;ZHAO Yueju;LIU Yang(Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process,Ministry of Agricultrue and Rural Affairs,Beijing 100193)

机构地区：[1]中国农业科学院农产品加工研究所/农业农村部农产品质量安全收贮运管控重点实验室,北京100193

出　　处：《核农学报》2020年第7期1507-1517,共11页Journal of Nuclear Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFC1600905)。

摘　　要：为了丰富玉米赤霉烯酮(ZEN)解毒菌种库,通过采集湖南省邵阳市等7个省市粮食样品,筛选获得1株能够高效降解ZEN的解淀粉芽孢杆菌NS2。采用高效液相色谱法检测降解前后ZEN的含量发现,液态发酵72 h,该菌对5 mg·L-1 ZEN标准品的降解率高达96.0%,固态发酵72 h,菌株对玉米粉中2.5 mg·L-1 ZEN标准品的降解率高达88.2%。采用高效液相色谱与四级杆飞行时间质谱联用技术检测该菌处理后的ZEN降解产物,未检测出具有毒性作用的α-玉米赤霉烯醇(α-ZOL)、β-玉米赤霉烯醇(β-ZOL)等ZEN的类似物。此外,抗菌活性试验与木聚糖酶、纤维素酶和蛋白酶活性试验显示降解菌NS2还能通过产生具有抗菌作用的次生代谢产物,抑制病原微生物大肠杆菌和鼠伤寒沙门氏菌的生长。上述结果表明,解淀粉芽孢杆菌NS2可以高效降解ZEN,具有潜在应用前景,为ZEN解毒产品的开发与应用奠定了材料基础。In order to enrich the zearalenone detoxification strain bank, a zearalenone-degrading strain NS2 was obtained and screened from food samples collected in 7 cities, such as Shaoyang City, Hunan Province, which was identified as Bacillus amyloliquefaciens by 16 s rDNA. The detection results of ZEN content before and after degration by high performance liquid chromatography showed that the degradation rate of the 5 mg·L-1 ZEN standard was as high as 96.0% and the solid fermentation was 72 h. The degradation rate of the strain on the 2.5 mg·L-1 ZEN standard in corn flour was as high as 88.2%. The ZEN degradation products treated by the bacteria were detected by high performance liquid chromatography and quadrupole time of flight mass spectrometry. No toxic effects of α-zearalenol(α-ZOL) and β-zearalenol(β-ZOL) were detected. an analog of estrogen ZEN. In addition, NS2 can inhibit the growth of pathogenic microorganisms Escherichia coli and Salmonella typhimurium by producing secondary metabolites with antibacterial action, according to antibacterial acticity test and xylanase, cellulase, proteuse activity test. These results indicate that Bacillus amyloliquefaciens NS2 can degrade ZEN efficiently, which has potential application prospects and lays a material foundation for the development and application of ZEN detoxification products.

关 键 词：玉米赤霉烯酮 降解 解淀粉芽孢杆菌 

分 类 号：S816.7[农业科学—饲料科学]