Fzd2诱导ER阴性乳腺癌细胞上皮-间质转化  

Fzd2 induces epithelial-mesenchymal transition in ER-negative breast cancer

作　　者：尹萍 王巍[1] 白瑜 那磊 赵成海[1] Yin Ping;Wang Wei;Bai Yu;Na Lei;Zhao Chenghai(Department of Pathophysiology,College of Basic Medical Science,China Medical University,Liaoning Shenyang 110122,China)

机构地区：[1]中国医科大学基础医学院病理生理学教研室,辽宁沈阳110122

出　　处：《现代肿瘤医学》2020年第16期2740-2744,共5页Journal of Modern Oncology

基　　金：辽宁省自然科学基金(编号:2019-MS-363);辽宁省重点研发计划指导计划项目(编号:2017225028)。

摘　　要：目的:分析Frizzled 2(Fzd2)在雌激素受体阴性(ER^-)乳腺癌中的表达及其对ER^-乳腺癌细胞上皮-间质转换(epithelial-mesenchymal transition,EMT)的影响。方法:采用免疫组化方法检测Fzd2在乳腺癌组织中的表达;采用Western blot检测Fzd2、E-cadherin、Vimentin、Slug、Zeb1在乳腺癌细胞中的表达;采用伤口愈合试验和Transwell试验检测细胞迁移和侵袭。结果:Fzd2在ER^-乳腺癌组织及细胞系中过表达;Fzd2敲减促进Hs578T细胞表达E-cadherin,减少Hs578T细胞表达Vimentin、Slug和Zeb1;Fzd2敲减抑制Hs578T细胞迁移和侵袭。结论:Fzd2诱导ER^-乳腺癌细胞发生EMT;Fzd2可能作为ER^-乳腺癌防治的一个潜在靶向分子。Objective:To investigate the expression of Frizzled 2(Fzd2)in ER-negative(ER-)breast cancer,and to explore the effect of Fzd2 on the epithelial-mesenchymal transition(EMT)in ER-breast cancer cells.Methods:Immunohistochemistry was used to detect the expression of Fzd2 in ER-and ER+breast cancer tissues.Western blot was used to examine the expression of Fzd2,E-cadherin,Vimentin,Slug and Zeb1 in breast cancer cells.Wound healing test and Transwell test were performed to analyze breast cancer cell migration and invasion.Results:Fzd2 was overexpressed in ER-breast cancer tissues and cells.Fzd2 knockdown enhanced the expression of E-cadherin,while reduced the expression of Vimentin,Slug and Zeb1 in Hs578T cells.Fzd2 knockdown suppressed migration and invasion of Hs578T cells.Conclusion:Fzd2 induces EMT in ER-breast cancer cells,and Fzd2 may be a potential target for prevention and therapy of ER-breast cancer.

关 键 词：Frizzled 2 ER^-乳腺癌 上皮-间质转换 

分 类 号：R737.9[医药卫生—肿瘤]