SRSF1对人膀胱癌BIU-87细胞增殖的作用及吡柔比星对SRSF1表达的影响  

Effect of SRSF1 on the proliferation of bladder cancer BIU-87 cells and the effect of pirarubicin on the expression of SRSF1

作　　者：于柳 刘屹立[1] YU Liu;LIU Yili(Department of Urology,the Fourth Affiliated Hospital of China Medical University,Shenyang 100032,China)

机构地区：[1]中国医科大学附属第四医院泌尿外科,沈阳100032

出　　处：《中华实用诊断与治疗杂志》2020年第7期656-659,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：辽宁省重点研发计划指导计划项目(2017225018)。

摘　　要：目的探讨丝氨酸/富含精氨酸剪接因子(serine/arginine-rich splicing factor 1, SRSF1)对人膀胱癌BIU-87细胞增殖的作用,分析吡柔比星对BIU-87细胞SRSF1表达的影响。方法对数生长期人膀胱癌BIU-87细胞随机分为空白对照组(不转染)、阴性对照组(转染siRNA-NC)、SRSF1干扰组(转染siRNA-SRSF1),采用Western blot法检测3组转染6 h时SRSF1相对表达量,CCK-8法检测3组转染后培养24、48 h细胞增殖。取对数生长期空白对照组细胞,随机分为对照组、0.4 mg/L吡柔比星组、0.8 mg/L吡柔比星组、1.6 mg/L吡柔比星组、3.2 mg/L吡柔比星组,分别加入吡柔比星浓度为0、0.4、0.8、1.6、3.2 mg/L的培养液1 mL培养24 h,采用Western blot法检测5组SRSF1蛋白相对表达量。结果转染6 h,SRSF1干扰组SRSF1蛋白相对表达量(0.070±0.053)低于空白对照组(0.692±0.069)、阴性对照组(0.776±0.088)(P<0.05),空白对照组与阴性对照组比较差异无统计学意义(P>0.05)。转染后培养24、48 h,SRSF1干扰组细胞增殖吸光度值(1.624±0.265、2.025±0.314)低于空白对照组(2.071±0.014、3.145±0.104)、阴性对照组(2.332±0.053、3.234±0.158)(P<0.05),空白对照组与阴性对照组比较差异无统计学意义(P>0.05)。SRSF1蛋白相对表达量在0.4 mg/L吡柔比星组(1.672±0.055)、0.8 mg/L吡柔比星组(1.963±0.028)、1.6 mg/L吡柔比星组(1.432±0.026)均高于对照组(1.036±0.065)(P<0.05),3.2 mg/L吡柔比星组(0.996±0.085)与对照组比较差异无统计学意义(P>0.05),0.4 mg/L、0.8 mg/L、1.6 mg/L、3.2 mg/L吡柔比星组SRSF1蛋白相对表达量呈先增高后降低趋势,峰值在0.8 mg/L吡柔比星组(P<0.05)。结论抑制SRSF1表达可抑制人膀胱癌BIU-87细胞增殖,低浓度吡柔比星可促进BIU-87细胞SRSF1表达,高浓度吡柔比星可抑制BIU-87细胞SRSF1表达。Objective To investigate the effect of serine/arginine-rich splicing factor 1(SRSF1)on the proliferation of human bladder cancer BIU-87 cells,and to analyze the effect of pirarubicin on the expression of SRSF1.Methods The human bladder cancer BIU-87 cells in logarithmic growth phase were randomly divided into blank control group(not transfected),negative control group(transfected with NC-small interfering RNA),and SRSF1 interfering group(transfected with SRSF1 expressing small interfering RNA).The relative expression of SRSF1 was detected by Western blot in three groups after 6 h of transfection,and the cell proliferation rates were detected by CCK-8 in 24 and 48 h of culture after transfection.The cells in blank control group in logarithmic growth phase were randomly divided into control group,0.4 mg/L pirarubicin group,0.8 mg/L pirarubicin group,1.6 mg/L pirarubicin group,and 3.2 mg/L pirarubicin group,adding with 1 mL culture medium with pirarubicin concentrations of 0,0.4,0.8,1.6,and 3.2 mg/L to culture for 24 h.The relative expression of SRSF1 protein in each group was detected by Western blot.Results After 6 h of transfection,the relative expression of SRSF1 protein was lower in SRSF1 interfering group(0.070±0.053)than that in blank control group(0.692±0.069)and negative control group(0.776±0.088)(P<0.05),and showed no significant difference between blank control group and negative control group(P>0.05).In 24 and 48 h of culture after transfection,the optical density values of cell proliferation were lower in SRSF1 interfering group(1.624±0.265,2.025±0.314)than those in blank control group(2.071±0.014,3.145±0.104),negative control group(2.332±0.053,3.234±0.158)(P<0.05),and showed no significnat differences between blank control group and negative control group(P>0.05).The relative expression of SRSF1 protein was higher in 0.4 mg/L pirarubicin group(1.672±0.055),0.8 mg/L pirarubicin group(1.963±0.028)and 1.6 mg/L pirarubicin group(1.432±0.026)than that in control group(1.036±0.065)(P<0.05),and s

关 键 词：膀胱癌 丝氨酸/富含精氨酸剪接因子 BIU-87细胞 细胞增殖 选择性剪接 

分 类 号：R737.14[医药卫生—肿瘤]