灵芝属多糖对肾上腺酮诱导神经细胞凋亡的保护作用  

Study on neuropreotection function of polysaccharide extracts from Ganoderma spp.

作　　者：赵爽[1] 许松琛 马传贵 宋爽[1] 苏哲 刘宇[1] ZHAO Shuang;XU Song-chen;MA Chuan-gui;SONG Shuang;SU Zhe;LIU Yu(Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Science,Beijing Engineering Research Center for Edible Mushroom,Beijing 100097,China;Beijing No.35 High School,Beijing 100032,China;Beijing Jingcheng Biotechnology Co,Ltd,Beijing 102600,China)

机构地区：[1]北京市农林科学院植物保护环境保护研究所,北京市食用菌工程技术研究中心,北京100097 [2]北京第三十五中学,北京100032 [3]北京京诚生物科技有限公司,北京102600

出　　处：《生物技术》2020年第3期269-274,共6页Biotechnology

基　　金：北京市农林科学农产品加工与营养健康协同创新中心(KJCX201915);北京市农林科学院科技创新能力建设专项(能量调控食品的研发2020-2022);北京市农林科学院农业科技示范推广项目(2019033)。

摘　　要：[目的]筛选和评价灵芝属多糖对肾上腺酮(CORT)诱导神经损伤的保护作用,为灵芝在抗抑郁功能方面的开发奠定基础。[方法]以CORT诱发神经细胞发生损伤后给予灵芝属多糖提取物,分别通过MTT法、乳酸脱氢酶(LDH)胞外释放法、总DNA定量分析法以及荧光标记法确定细胞毒性以及对损伤细胞的保护作用。[结果]结果显示灵芝属多糖在低作用浓度下对PC12细胞不具有毒性,在100μg/mL的作用浓度下灵芝孢子粉多糖(PGL)可以显著提高损伤细胞存活率的比例为22%,减少LDH的释放比例为11.08%,增加胞内总DNA含量14.65%,是所有待测多糖中作用最佳的。[结论]PGL对细胞具有低毒的特点,显著改善CORT对细胞的损伤作用,具有明显的神经保护作用,可作为抗抑郁药物进行研究和开发。[Objective]This study investigated that the neuroprotection effects of polysaccharide extracts of Ganoderma spp.in corticosterone-induced cell model,which provided the scientific data for the development and application of Ganoderma spp.in antidepressant medicine area.[Method]The nerve injury model was built by corticosterone-induction,and the dosages of Ganoderma spp.polysaccharides were treated in the same concentration.The cytotoxicity and protection effects were detected using MTT method,a lactate dehydrogenase(LDH)assay kit,total DNA quantification and fluorescence labeling method.[Result]The results showed that the polysaccharide extracts of Ganoderma spp.were not toxic towards PC12 at the low concentrations.The polysaccharide of Ganoderma lucidum spores at the concentration of 100μg/mL improved the injured cell viability with the ratio of 22%,reduced the release of LDH with the ratio of 11.08%,increased the amount of total DNA with the ratio of 14.65%.The PGL was the advanced the polysaccharide of Ganoderma spp.extracts.[Conclusion]PGL could recover the injury effect of CORT,and protect the injured nerve cells without cytotoxicity,which can be explored in the area of antidepressant therapy.

关 键 词：赤芝 紫芝 西藏白肉灵芝 灵芝孢子粉 神经保护作用 

分 类 号：R285.5[医药卫生—中药学]