两种酒精灌胃方案诱导大鼠酒精性肝损伤模型的比较研究  

Comparison of Two Rat Models of Alcoholic Liver Injury Induced by Gradient Alcohol Gavage or Fixed Alcohol Gavage

作　　者：胡昊 李宁 宋圆圆 王博 龚志强 杨艳 HU Hao;LI Ning;SONG Yuanyuan(Preventive Medicine Undergraduate,School of Public Health,Southwest Medical University,Luzhou City,Sichuan Province 646000;不详)

机构地区：[1]西南医科大学公共卫生学院,四川省泸州市646000 [2]西南医科大学公共卫生学院卫生检验与检疫教研室

出　　处：《医学理论与实践》2020年第15期2420-2422,共3页The Journal of Medical Theory and Practice

基　　金：四川省大学生创新创业训练计划项目(201710632076)。

摘　　要：目的:筛选一种简便、稳定、可靠的酒精性肝损伤模型。方法:通过白酒灌胃8周建立酒精性肝损伤大鼠模型。依据两种灌胃方案分成两组:一种固定10ml/kg的剂量灌胃,称固定酒精灌胃组;一种以7ml/kg剂量灌胃为基础,每2周按2ml/kg递增,称梯度酒精灌胃组。两组大鼠灌胃的酒精平均剂量相等,总量相当。实验期间记录大鼠的死亡情况和体重变化,实验结束后分析血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性和甘油三酯(TG)、总胆固醇(TC)含量,肝脏丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,取肝组织HE染色进行病理学分析。结果:造模8周后,两种方案均诱导大鼠肝细胞肿胀和脂肪组织沉积等病理变化,体重增长受到抑制,血清ALT、AST、TG、TC及肝脏MDA升高,肝脏SOD降低(P<0.05),提示出现明显肝损伤。与固定酒精灌胃组比较,梯度酒精灌胃组大鼠血清ALT、AST、TG、TC,肝脏MDA升高,肝脏SOD降低,且酒精敏感死亡率低。结论:梯度酒精灌胃模型优于固定酒精灌胃模型。梯度酒精灌胃模型相对固定酒精灌胃模型更适合用于酒精性肝损伤分子机制的研究和防治药物的筛选。Objective:To select a simple,stable and reliable rat model of alcoholic liver injury.Methods:The rat models of alcoholic liver injury were induced by high-alcohol liquor gavage for 8 weeks.One group was fed with dose of 10ml/kg,referred to as the fixed alcohol gavage group.The other group was based on dose of 7ml/kg and increasing by 2ml/kg every 2 weeks,referred to as the gradient alcohol gavage group.The average dose and total amount of alcohol in the two groups were equal.Death and weight changes of rats were recorded during the experiment.After the rats were sacrificed,the activities of alanine transaminase(ALT)and aspartate transaminase(AST),the content of triglyceride(TG)and total cholesterol(TC)in serum,and the activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in liver were analyzed.Liver tissues were stained with HE for pathological analysis.Results:After modeling,both of the two schemes induced pathological changes such as liver cell swell and adipose tissue deposition.Weight gain of rats was inhibited.ALT,AST,TG,TC in serum and MDA in liver increased while SOD in liver decreased(P<0.05),which indicated obvious liver injury.Compared with the fixed group,ALT,AST,TG,TC in serum and MDA in liver increased and SOD in liver decreased in the gradient group,and the alcohol sensitive mortality of rats was lower.Conclusion:The gradient alcohol gavage model is better than the fixed alcohol gavage model,and it is more suitable to study the molecular mechanism of alcoholic liver injury as well as to screen preventive and therapeutic drugs.

关 键 词：酒精性肝损伤 酒精灌胃 动物模型 大鼠 

分 类 号：R-33[医药卫生]