热浸提-高效液相色谱等度分离-原子荧光光谱法测定水产品中的4种砷形态  

Determination of four arsenic species in aquatic products by thermal extraction-high performance liquid chromatography-isocratic separation-atomic fluorescence spectrometry

作　　者：韦丽丽[1] 石莉莉[1] 覃月媚 WEI Li-li;SHI Li-li;QIN Yue-mei(Wuzhou Center for Disease Prevention and Control,Wuzhou 543002,China)

机构地区：[1]梧州市疾病预防控制中心,广西梧州543002

出　　处：《现代化工》2020年第7期240-243,共4页Modern Chemical Industry

摘　　要：建立了水产品中4种砷形态的热浸提-高效液相色谱等度分离-原子荧光光谱分析法,并对流动相浓度及pH、流速、载流浓度、泵速、硼氢化钾浓度等条件进行了优化。在最佳实验条件下,亚砷酸[As(Ⅲ)]、一甲基砷(MMA)、二甲基砷(DMA)和砷酸[As(Ⅴ)]4种砷形态在5~100μg/L范围内线性关系良好,相关系数大于0.9995,检出限在0.01~0.03 mg/kg,相对标准偏差(RSD,n=7)在1.5%~5.0%。同时对不同样品进行3个水平的加标回收实验,各组分的加标回收率在84.0%~97.5%。该方法操作简便,准确度好,精密度高,可用于测定水产品中的砷形态。Thermal extraction-high performance liquid chromatography-isocratic separation-atomic fluorescence spectrometry method is developed for the determination of four arsenic species in aquatic products.Various conditions are optimized,such as the concentration,pH value and flow velocity of the mobile phase,carrier current concentration,pump speed and potassium borohydride concentration.Under the optimal conditions,there are good linear relationships for arsenious acid[As(Ⅲ)],arsenic acid[As(Ⅴ)],monomethylarsenic acid(MMA),dimethylarsenic acid(DMA)in the range of 5-100μg·L-1 with correlation coefficients(r2)larger than 0.9995,detection limits being in the range of 0.01-0.03 mg·kg-1,and relative standard deviations remaining between 1.5%and 5.0%.The average recoveries at three spiked levels are in the range of 84.0%-97.5%.This method exhibits simple,good accuracy and height sensitivity,and can be applied in the species analysis of arsenic in aquatic products.

关 键 词：砷形态 水产品 高效液相色谱等度分离-原子荧光光谱法 

分 类 号：TH3[机械工程—机械制造及自动化]