老年溃疡性结肠炎患者肠黏膜和外周血中miR-215的表达及意义  

Expression and significance of miR-215 in intestinal mucosa and peripheral blood of elderly patients with ulcerative colitis

作　　者：田徐露 田小兰[2] 黄奕君[1] 岑运光[1] 王太昊[1] 崔晓燕 TIAN Xu-lu;TIAN Xiao-lan;HUANG Yi-jun;CEN Yun-guang;WANG Tai-hao;CUI Xiao-yan(Medical and Health Care Center of Hainan General Hospital,Haikou,Hainan 570311,China;不详)

机构地区：[1]海南省人民医院医疗保健中心,海南海口570311 [2]海南省人民医院消化科,海南海口570311

出　　处：《中国临床研究》2020年第7期927-930,共4页Chinese Journal of Clinical Research

摘　　要：目的探讨老年溃疡性结肠炎患者肠黏膜和外周血中微小核糖核酸-125(miR-215)的表达及临床意义。方法采用回顾性研究方法,选择2017年1月至2018年1月消化内科确诊的老年溃疡性结肠炎患者98例为病例组,选择同期老年结肠息肉患者86例作为对照组。采用qRT-PCR法测定肠黏膜和外周血中miR-215表达水平;流式细胞仪检测外周血CD44、辅助性T细胞(Th)17占总淋巴细胞的比例;酶联免疫吸附法测定外周血白细胞介素(IL)-4、IL-10、IL-12、肿瘤坏死因子(TNF)-α、超敏C反应蛋白(hs-CRP)水平。结果病例组肠黏膜和外周血的miR-215水平高于对照组(P均<0.01),且随着溃疡性结肠炎严重程度的增加(Ⅰ级→Ⅱ级→Ⅲ级),肠黏膜和外周血miR-215水平逐渐增加(P均<0.01)。病例组CD44、IL-4、IL-10、IL-12、TNF-α、hs-CRP、Th17水平高于对照组(P均<0.01)。肠黏膜和外周血的miR-215表达水平与CD44、IL-4、IL-8、IL-10、TNF-α、hs-CRP、TH17分别呈正相关(P<0.05,P<0.01)。高水平肠黏膜miR-215[OR=2.49,95%CI(1.13~5.23),P=0.013]和外周血miR-215[OR=2.21,95%CI(1.06~4.55),P=0.016]均为老年溃疡性结肠炎治疗无效的危险因素。结论老年溃疡性结肠炎患者肠黏膜和外周血中miR-215表达水平升高,且与疾病严重程度呈正相关;miR-215可能通过促进炎症因子的释放加剧疾病的进展;高水平肠黏膜和外周血的miR-215均为老年溃疡性结肠炎治疗无效的危险因素。Objective To investigate the expression and significance of microRNA-215(miR-125)in intestinal mucosa and peripheral blood of elderly patients with ulcerative colitis.Methods Using retrospective analysis,98 elderly patients with ulcerative colitis were selected as the case group,and 86 elderly patients with colonic polyps were selected as the control group at the same time.The expression of miR-215 in intestinal mucosa and peripheral blood was measured by qRT-PCR,the proportion of CD44 and Th17 cells in peripheral blood to total lymphocyte was detected by flow cytometry,and the levels of IL-4,IL-10,IL-12,TNF-αand hs-CRP in peripheral blood were measured by ELISA.Results The levels of miR-215 in intestinal mucosa and peripheral blood in the case group were higher than those in the control group(all P<0.01).With the increase of the severity of ulcerative colitis(gradⅠ→gradeⅡ→gradeⅢ),the levels of miR-215 in intestinal mucosa and in peripheral blood were gradually increased(all P<0.01).The levels of CD44,IL-4,IL-10,IL-12,TNF-α,hs-CRP and Th17 in the case group were significantly higher than those in the control group(all P<0.01).miR-215 in intestinal mucosa and in peripheral blood were positively correlated with CD44,IL-4,IL-8,IL-10,TNF-α,hs-CRP and Th17,respectively(P<0.05,P<0.01).High levels of intestinal mucosal miR-215[OR=2.49,95%CI(1.13-5.23),P=0.013]and peripheral blood miR-215[OR=2.21,95%CI(1.06-4.55),P=0.016]were risk factors for ineffective treatment of ulcerative colitis in the elderly.Conclusions The expression level of miR-215 in intestinal mucosa and peripheral blood of elderly patients with ulcerative colitis is increased,and it was positively correlated with the severity of the disease.miR-215 may aggravate the progress of ulcerative colitis in the elderly by promoting the release of inflammatory factors.High level of miR-215 in intestinal mucosa and in peripheral blood are risk factors for ineffective treatment of the disease.

关 键 词：老年 溃疡性结肠炎 肠黏膜 外周血 微小核糖核酸-125 炎性因子 

分 类 号：R574.1[医药卫生—消化系统]