浸润性乳腺癌中IGF-1/IGF-1R的表达方式及对表阿霉素治疗的生物学意义  

Expression and Response of IGF-1/IGF-1R in Invasive Breast Cancer with Doxorubicin Treatment

作　　者：顾丽琴[1] 刘文婷[2,3] 顾国建 包健荣[1] 杜欢 黄梦洁 谢芳 GU Li-qin;LIU Wen-ting;GU Guo-jian;BAO Jian-rong;DU Huan;HUANG Men-jie;XIE Fang(Department of Pathology,Taicang Hospital of Traditional Chinese Medicine,Taicang,Jiangsu,215400,China;Department of Pathophysiology and Pathology,Soochow University Medical College,Suzhou,Jiangsu,215123,China;Department of Pathology,the First Affiliated Hospital of Soochow University,Suzhou,Jiangsu,215006,China;Department of Pathology,Taicang No.1 People’s Hospital,Taicang,Jiangsu,215400,China)

机构地区：[1]江苏省太仓市中医医院病理科,江苏太仓215400 [2]苏州大学基础医学与生物科学学院病理与病理生理学系,江苏苏州215123 [3]苏州大学附属第一医院病理科,江苏苏州215006 [4]江苏省太仓市第一人民医院病理科,江苏太仓215400

出　　处：《中国血液流变学杂志》2019年第4期388-393,共6页Chinese Journal of Hemorheology

基　　金：国家自然科学基金资助项目(81772809);太仓市科技计划项目(TC2017SFYL12)。

摘　　要：目的探讨IGF-1/IGF-1R蛋白在浸润性乳腺癌组织及细胞中的表达和对表阿霉素治疗的生物学意义.方法采用免疫组化GTVSIONTMⅢ二步法检测IGF-1/IGF-1R蛋白在165例乳腺肿瘤组织中的表达及其与临床病理参数间的关系;利用Western Blot方法检测乳腺癌细胞株经表阿霉素治疗后,IGF-1及IGF-1R蛋白的表达情况.结果(1)浸润性乳腺癌组织中IGF-1、IGF-1R阳性表达明显高于导管内原位癌及纤维腺瘤组织(P<0.05).(2)浸润性乳腺癌组织中IGF-1R膜表达随肿瘤恶性程度的升高而增加(P<0.05).(3)表阿霉素治疗后,乳腺癌细胞株中IGF-1蛋白的表达升高.结论IGF-1R的异常表达与浸润性乳腺癌的恶性演变有关,IGF-1/IGF-1R信号与化学治疗有一定关系.Objective To investigate the expressions of IGF-1 and IGF-1R protein in breast cancer and its relationship with the clinical characteristics.Methods The expressions of IGF-1/IGF-1R were examined by immunohistochemical method.And the response of IGF-1 and IGF-1R protein in breast cancer cell lines during DOX treatment was analyzed by Western Blot.Results(1)The expression of IGF-1 and IGF-1R in breast cancer were higher than that in fibroadenoma and in ductal carcinoma in situ(P<0.05).(2)The positive expression of IGF-1R in cytomembrane was significantly associated with increasing tumor grade in invasive breast cancer(P<0.05).(3)The expression of IGF-1 protein in breast cancer cell lines was increased due to with DOX treatment.C onclusion The upregulation of expression of IGF-1 R and signal of IGF-1/IGF-1R may take part in the carcinogenesis and treatment of invasive breast cancer.

关 键 词：IGF-1 IGF-1R 浸润性乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤] R730.2[医药卫生—临床医学]