基于免疫组化法研究MMP-2、MMP-14与颈动脉粥样斑块易损性的相关性  

Study on the relationship between MMP-2,MMP-14 and the vulnerability of carotid plaque based on immunohistochemistry

作　　者：郭周颖 张淑琴[1] 秦颢诚 于明[1] 李洪娥[1] GUO Zhouying;ZHANG Shuqin;QING Haocheng(Department of Ultrasonography,the First People’s Hospital of Lianyungang,Lianyungang 222002,China)

机构地区：[1]连云港市第一人民医院,江苏连云港222002

出　　处：《中风与神经疾病杂志》2020年第7期580-584,共5页Journal of Apoplexy and Nervous Diseases

基　　金：江苏省六大人才高峰项目(No.WSW-045)。

摘　　要：目的研究基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2),MMP-14与颈动脉粥样斑块易损性的关系,探讨影响斑块稳定性的相关因素。方法选取2018年1月~2019年6月在连云港市第一人民医院神经外科行颈动脉内膜剥脱术(endarterectomy,CEA)患者55例。术前根据基于颈部血管超声(carotid doppler ultrasonography,CDU)的多模式影像学检查、术后粥样斑块HE染色特征,将粥样斑块组织分为稳定斑块组23例及易损斑块组32例。应用免疫组织化学法定位分析MMP-2及MMP-14在稳定斑块组及易损斑块组中表达的分布差异。结果免疫组化结果显示MMP-2,MMP-14在颈动脉粥样斑块中表达分布,其在易损斑块组中分布水平明显高于稳定斑块组(P<0.05),且在溃疡型斑块,斑块纤维帽破裂等处分布较高。结论MMP-2和MMP-14在颈动脉粥样斑块中的表达水平与斑块易损性存在显著相关性,是影响斑块稳定性的重要因子。Objective We investigated the correlation of MMP-2,MMP-14 with the vulnerability of carotid plaque.Our study was to explore the related factors which influence the stability of the plaques.Methods Carotid plaques were collected from patients undergoing CEA in the Department of Neurosurgery of the First people’s Hospital of Lianyungang.55 patients were recruited between January 2018 and June 2019.All of the plaques were divided into 23 stable carotid plaques and 32 vulnerable carotid plaques by imaging and histological analysis.Immunohistochemistry was used toloca lize the expression level of MMP-2 and MMP-14 in stable and vulnerable plaques.Results Density analysis revealed that the immunohistochemical staining of MMP-2 and MMP-14 were more intense among samples from vulnerable group than those from stable group.MMP-2 and MMP-14 staining was positive in ulcer plaque and the atheromatous lesions.Conclusion The expression of MMP-2 and MMP-14 were associated with plaque vulnerability of human carotid plaques,which is an important factor affecting the stability of plaques.

关 键 词：颈动脉斑块 血管超声 基质金属蛋白酶 易损性 相关性 

分 类 号：R743[医药卫生—神经病学与精神病学]