ELF5通过抑制上皮间质转化抑制结直肠癌的发生发展  

The Suppression of the Progress of Colorectal Cancer by ELF5 Through Inhibiting Epithelial-Mesenchymal Transition

作　　者：马骏[1] 陈树军[1] 秦阳[1] 张玉英[2] MA Jun;CHEN Shujun;QIN Yang;ZHANG Yuying(General Surgery Four Branches,Affiliated Central Hospital of Shenyang Medical College,Shenyang 110024,China;Radiology Department,Affiliated Central Hospital of Shenyang Medical College,Shenyang 110024,China)

机构地区：[1]沈阳医学院附属中心医院普外四科,辽宁沈阳110024 [2]沈阳医学院附属中心医院放射科,辽宁沈阳110024

出　　处：《标记免疫分析与临床》2020年第7期1213-1218,共6页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨ELF5在结直肠癌发生发展中的作用。方法 Real-time PCR及Western blot法检测结直肠癌组织及细胞株中ELF5表达变化,细胞转染技术过表达结肠癌细胞中ELF5,CCK-8试验检测细胞增殖能力,细胞划痕实验检测细胞迁移能力,transwell小室实验检测细胞侵袭能力,细胞免疫荧光及Western blot法检测E-cadherin、Vimentin、N-cadherin和Snail蛋白表达变化,评估EMT发生情况。结果结肠癌组织及细胞株中ELF5表达较对照组明显下降(P<0.001)。过表达结肠癌细胞中ELF5可抑制细胞的增殖、迁移及侵袭(P<0.05),且可抑制EMT的发生(P<0.05)。结论 ELF5可能通过抑制EMT发生进而抑制结直肠癌的发生发展。Objective To investigate the role of ELF5 in colorectal cancer.Methods The expression of ELF5 in colorectal cancer tissues and cell lines were detected by real-time PCR and western blot. After overexpressed ELF5, the ability of proliferation, migration and invasion of CaCo2 cells were detected by CCK-8, cell scratch assay and Transwell chamber assay, respectively. Immunofluorescence and western blot were used to detect the expressions of E-cadherin, Vimentin, N-cadherin and Snail.Results The expression of ELF5 was reduced in CRC(P<0.001). Overexpression of ELF5 suppressed CaCo2 cells′ ability of proliferation, migration and invasion(P<0.05), and inhibited EMT(P<0.05).Conclusion ELF5 suppresses the progress of colorectal cancer by inhibiting EMT.

关 键 词：ELF5 上皮间质转化 结直肠癌 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]