MiRNA-92a靶向PTEN通过PI3K/AKT信号通路促进卵巢癌细胞转移  

MiRNA-92a promote metastasis in ovarian cancer cells through PI3K/Akt signaling pathway by targeting PTEN

作　　者：王秋宇[1] 朱军义[1] 邓巧子[2] WANG Qiuyu;ZHU Junyi;DENG Qiaozi(Department of Obstetrics and Gynecology,Nanyang City Center Hospital,Nanyang Henan 473000;Department of Obstetrics and Gynecology,The First Affiliated Hospital of Henan University of Science and Technology,Luoyang Henan 471000,China)

机构地区：[1]南阳市中心医院妇产科,河南南阳473000 [2]河南科技大学第一附属医院新区医院妇产科,河南洛阳471000

出　　处：《临床与病理杂志》2020年第7期1658-1667,共10页Journal of Clinical and Pathological Research

基　　金：洛阳市应用技术研究与开发资金项目(1201052A2)。

摘　　要：目的:探讨miRNA-92a对卵巢癌细胞侵袭与迁移能力变化的影响和可能的作用机制。方法:RTqPCR检测卵巢癌组织和卵巢癌细胞miRNA-92a的表达水平;转染miRNA-92ainhibitor/mimic后,Transwell小室法检测SKOV3与A2780细胞的侵袭与迁移能力;蛋白质印迹法检测SKOV3与A2780细胞中p-PI3k,p-Akt和PTEN蛋白水平的变化;预测miRNA-92a靶基因并经双荧光素酶报告基因验证。结果:MiRNA-92a在卵巢癌组织和卵巢癌细胞中高表达(P<0.05);转染miRNA-92a inhibitor后,SKOV3与A2780细胞的侵袭和迁移能力明显受到抑制,同时p-PI3k和p-Akt蛋白水平均显著降低,PTEN蛋白水平显著升高(均P<0.05);转染miRNA-92a mimic后,以上指标相反;miRNA-92a靶基因为PTEN,且过表达PTEN能逆转过表达miRNA-92a诱导的侵袭转移与p-PI3k,p-Akt表达(均P<0.05)。结论:MiRNA-92a直接结合靶基因PTEN,通过激活PI3k/Akt通路活性,促进卵巢癌细胞SKOV3的侵袭与迁移能力。Objective:To investigate the effects and the possible mechanisms of miRNA-92a on the abilities of cell invasion and migration in ovarian cancer cells.Methods:The expressions of miRNA-92a in the ovarian cancer tissues and ovarian cancer cells were detected by RT-qPCR.After transfection with miRNA-92a inhibitor or mimic,the abilities of SKOV3 and A2780 cell invasion and migration were determined by Transwell assay.The protein levels of p-PI3K,p-Akt and PTEN were measured by Western blotting.Further,miRNA-92a target gene was predicted and then be verified by dual-luciferase reporter system assay.Results:The expressions of miRNA-92a were significantly higher in the ovarian cancer tissue and ovarian cancer cells(P<0.05).After transfected with miRNA-92a inhibitor into SKOV3 and A2780 cells,the abilities of invasion and migration were dramatically reduced,the protein levels of p-PI3K and p-Akt were markedly decreased and PTEN was markedly increased(all P<0.05).However,after transfection with miRNA-92a mimic,the above indicators were contrary.Furthermore,PTEN was the direct target gene of miRNA-92a,and over-expression PTEN reversed the migration and invasion induced by over-expression miRNA-92a in SKOV3 and A2780 cells,and the high expression of p-PI3k and p-Akt(all P<0.05).Conclusion:MiRNA-92a promotes the abilities of invasion and migration in the SKOV3 and A2780 ovarian cancer cells though activating of PI3K/Akt pathway by direct targeting PTEN.

关 键 词：卵巢癌 miRNA-92a 侵袭 迁移 

分 类 号：R737.31[医药卫生—肿瘤]