活络骨康丸治疗兔酒精性股骨头坏死的实验研究  

Observation of Huoluo Gukang Pills on the Treatment Alcohol-induced Osteonecrosis of the Femoral Head in Rabbits

作　　者：王国宾 王少华[2] 侯颖周[2] 李科伟[2] 白玉[2] 李志强[3] 李征 WANG Guobin;WANG Shaohua;HOU Yingzhou;LI Kewei;BAI Yu;LI Zhiqiang;LI Zheng(Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China;Joint Disease Department,Zhengzhou Orthopaedic Hospital,Zhengzhou 450052,China;Orthopaedics Department,Shangqiu First People's Hospital,Shangqiu 476100,Henan China)

机构地区：[1]河南中医药大学,郑州450008 [2]郑州市骨科医院关节科 [3]商丘市第一人民医院骨科

出　　处：《中国中医骨伤科杂志》2020年第7期6-10,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：郑州市科技攻关项目(153PKJGG083)

摘　　要：目的:探讨活络骨康丸治疗股骨头坏死的作用。方法:日本大耳白兔30只,随机分为三组。A组(实验组)10只采用灌胃法,给予烈性酒(酒精度46%)10 mL/(kg·d),灌胃90 d后停止,改用活络骨康丸0.84 g/(kg·d)灌胃60 d。B组(模型组)10只给予烈性酒(酒精度46%)10 mL/(kg·d)灌胃90 d后改为常规喂养60 d。N组(正常对照组)10只予10%葡萄糖溶液10 mL/(kg·d)持续灌胃150 d。三组抽血取材,测定血清低密度脂蛋白、甘油三酯和胆固醇含量,观察股骨头组织病理学变化。结果:A组软骨区可见软骨下潮线分离,骨小梁变细,骨松质髓腔数与N组差异无统计学意义(P>0.05);B组有软骨缺失,骨小梁大量断裂,骨松质髓腔数明显少于N组,差异有统计学意义(P<0.001)。A组三项指标(血清总胆固醇浓度、低密度脂蛋白浓度、甘油三酯浓度)均低于B组,差异有统计学意义(P<0.001);B组三项指标均高于N组,差异有统计学意义(P<0.05)。结论:活络骨康丸可降低血清胆固醇、低密度脂蛋白和甘油三酯的浓度,促进兔股骨头骨髓间充质干细胞成骨分化,抑制其成脂分化,防止骨质破坏吸收,延缓股骨头坏死的病程进展,活络骨康丸可用于治疗早期股骨头坏死。Objective:Study the effect of Huoluo Gukang pills on the treatment of alcohol-induced femoral head necrosis in rabbits.Methods:30 Japanese big-eared white rabbits(hereinafter referred to as rabbits)were randomly divided into 3 groups.10 rats in group A(experimental group)were administered with gavage by hard liquor(46%alcohol content,vol)10 mL/(kg·d)for 90 d and switched to Huoluo Gukang pills 0.84 g/(kg·d)gavage for 60 d.10 rats in group B(model group)were given hard alcohol(46%alcohol content,vol)10 mL/(kg·d)for 90 d and switched to normal gavage for 60 d.10 rats in the group N(normal control group)were continued to administer the gavage with 10%glucose solution 10 mL/(kg·d)for 150 d.Blood samples were collected from the three groups,the contents of serum LDL,TG and cholesterol were measured,and the histopathological changes of femoral head were observed.Results:In group A,the subchondral hygroscopic line was separated.The bone trabeculae became thin,and the number of bone cancellous medullary cavity had no statistical difference with that in group N(P>0.05).In group B there was cartilage deficiency.Bone trabeculae were broken in large quantity,and the number of bone cancellous medullary cavity was significantly less than that in group N(P<0.001).Serum total cholesterol concentration,LDL concentration,triglyceride concentration in group A were lower than group B(P<0.001),three indicators of group B were higher than these of group N(P<0.05).Conclusion:Huoluo Gukang pills can reduce the concentration of serum cholesterol,low density lipoprotein and triglyceride,promote osteogenic differentiation of bone marrow mesenchymal stem cells of femoral head of rabbits,inhibit adipogenic differentiation,prevent bone destruction and absorption,and delay the progress of femoral head necrosis.Huoluo Gukang pills can treat early necrosis of femoral head.

关 键 词：活络骨康丸 治疗 酒精 股骨头坏死 

分 类 号：R-33[医药卫生]