硫氧还蛋白2在老年白内障患者晶状体前囊膜中的表达及其与细胞凋亡、氧化应激的关系  

Expression of thioredoxin-2 in anterior capsule of lens and its relationship with apoptosis and oxidative stress in elderly cataract patients

作　　者：伍超[1] 杨旭[1] WU Chao;YANG Xu(Department of Ophthalmology, Hanzhong People′s Hospital, Shaanxi Province, Hanzhong 723000, China)

机构地区：[1]陕西省汉中市人民医院眼科,陕西汉中723000

出　　处：《河北医科大学学报》2020年第7期801-804,833,共5页Journal of Hebei Medical University

基　　金：陕西省教育厅自然科学研究项目(2013J1K0782)。

摘　　要：目的探讨硫氧还蛋白2(thioredoin-2,Trx-2)在老年白内障患者晶状体前囊膜中的表达及其与细胞凋亡、氧化应激的关系。方法选择年龄相关性白内障并行超声乳化术吸出白内障的老年患者40例40眼作为研究组,因外伤行透明晶状体取出术(非白内障)的老年患者20例20眼作为对照组。采用免疫组织化学法测定晶状体前囊膜组织中Trx-2的阳性表达情况。比较2组标本组织和研究组不同Trx-2(+)、Trx-2(-)标本组织中凋亡相关分子(P53、Bcl-2、ASK1)蛋白表达量、氧化应激指标[超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性及谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)]水平。结果研究组标本中Trx-2表达阳性率显著低于对照组标本(P<0.05)。研究组晶状体前囊膜标本中P53、ASK1的蛋白表达量高于对照组,Bcl-2的蛋白表达量低于对照组;标本上清液中SOD、CAT、GSH的水平低于对照组,MDA的水平高于对照组(P<0.01)。研究组中,Trx-2(+)的晶状体前囊膜标本中P53、ASK1的蛋白表达量低于Trx-2(-)者,Bcl-2的蛋白表达量高于Trx-2(-)者;标本上清液中SOD、CAT、GSH的水平高于Trx-2(-)者,MDA的水平低于Trx-2(-)者(P<0.05或P<0.01)。结论老年白内障患者晶状体前囊膜中Trx-2表达减弱,其抗凋亡及抗氧化应激作用减弱,可能是导致疾病发生发展的重要分子机制之一。Objective To investigate the expression of thioredoxin-2(Trx-2)in anterior capsule of lens and its relationship with apoptosis and oxidative stress in elderly cataract patients.Methods Forty cases 40 eyes of elderly cataract patients underwent phacoemulsification cataract extraction were chosen as study group,20 cases 20 eyes elderly patients treated with transparent lens extraction(non-cataract)due to trauma were chosen as control group.The positive expression of Trx-2 in anterior capsule of lens was determined by immunohistochemistry.Protein expression of apoptosis related molecules[P53 gene(P53),B-cell lymphoma-2(Bcl-2),Apoptosis signal-regulating kinase 1(ASK1)],contents of oxidative stress indexes[superoxide dismutase(SOD),catalase(CAT)activity and glutathione(GSH),malondialdehyde(MDA)]levels were compared between study group,control group,Trx-2(+)and Trx-2(-)specimens in study group.Results Positive rate of Trx-2 expression in study group was significantly lower than that in control group(P<0.05).Protein expressions of P53 and ASK1 in anterior capsular membrane of study group were higher than those in control group,while protein expressions of bcl-2 was lower than that in control group.Supernatant levels of SOD,CAT and GSH were lower than those in control group,and level of MDA was higher than that in control group(P<0.01).In study group,the protein expression levels of P53 and ASK1 in Trx-2(+)specimens were lower than those of Trx-2(-),protein expression level of bcl-2 was higher than that of Trx-2(-).levels of SOD,CAT and GSH in Trx-2(+)were higher than those in Trx-2(-),level of MDA was lower than that in Trx-2(-)(P<0.05 or P<0.01).Conclusion Expression of Trx-2 in anterior lens capsule of elderly cataract patients decreases,and its anti-apoptosis、anti-oxidative stress are weakened,which may be one of the important molecular mechanisms leading to the occurrence and development of the disease.

关 键 词：白内障 硫氧还蛋白2 细胞凋亡 氧化应激 

分 类 号：R776.1[医药卫生—眼科]