山药总皂苷体外抗氧化活性及质量控制方法考察  

In vitro Antioxidant Activity of Total Saponins of Dioscoreae Rhizoma and Its Quality Control

作　　者：平静[1] 毕开顺[2] Ping Jing;Bi Kaishun(Virus Laboratory,Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;College of Pharmacy,Shenyang Pharmaceutical University)

机构地区：[1]辽宁中医药大学附属医院病毒实验室,沈阳110032 [2]沈阳药科大学药学院

出　　处：《中国药师》2020年第7期1439-1442,共4页China Pharmacist

摘　　要：目的:考察山药总皂苷体外抗氧化活性,建立HPLC法同时测定山药总皂苷中薯蓣皂苷、原薯蓣皂苷含量,为山药总皂苷成分研究提供参考。方法:采用DPPH自由基、邻苯三酚超氧阴离子反应体系,以维生素C作为参照物,检测山药总皂苷的抗氧化活性。色谱柱:Kromasil-C18柱(250 mm×4.6 mm,5μm);流动相:乙腈(A)-水(B),梯度洗脱0~10 min,30%→40%A;10~14 min,40%→54%A;流速:1.0 ml·min^-1;检测波长:203 nm;柱温:30℃进行含量测定。结果:当山药总皂苷浓度为0.5mg·ml^-1时对超氧阴离子的抑制率为39.4%;对DPPH自由基清除率为31.6%。总皂苷中薯蓣皂苷、原薯蓣皂苷的色谱峰面积与浓度呈良好的线性关系,线性范围分别为52.40~838.00μg·ml^-1(r=0.9997),58.40~934.00μg·ml^-1(r=0.9998);平均回收率分别为96.1%、96.3%,RSD分别为1.8%,1.9%(n=9)。结论:表明山药总皂苷具有一定的清除DPPH自由基和超氧阴离子的活性,对其所建立的质量控制方法简便可行、重现性好,为山药总皂苷的质量评价提供方法。Objective:To investigate the antioxidant activity of total saponins of Dioscoreae Rhizoma and establish an HPLC method for the simultaneous determination of dioscin and protodioscin in total saponins of Dioscoreae Rhizoma.Methods:Antioxidant activity was studied by scavenging DPPH radical and pyrogallol superoxide anion free radical.The method of determination was performed on a Kromasil-C18 column(250 mm×4.6 mm,5μm)with the mobile phase consisting of acetonitrile(A)and water(B)with gradient elution at a flow rate of 1.0 ml·min^-1.The following gradient procedure was used:0-10 min,30%→40%A;10-14 min,40%→54%A.The detection wavelength was 203 nm,and the column temperature was 30℃.Results:The results showed that at the concentration of 0.5 mg·ml^-1,the inhibition rate of superoxide anion free radical was 39.4%,and the scavenging rate of DPPH radica1 was 31.6%.The method had good linear relationship within the range of 52.40-838.00μg·ml^-1 for dioscin(r=0.9997)and 58.40-934.00μg·ml^-1 for protodioscin(r=0.9998).The average recovery was 96.1%for dioscin and 96.3%for protodioscin,and the RSD was 1.8%and 1.9%(n=9),respectively.Conclusion:Total saponins extraction of Dioscoreae Rhizoma shows antioxidant activity to some extent.The method of determination is simple,accurate and reproducible.It provides a reliable way for the quality evaluation of total saponins of Dioscoreae Rhizoma.

关 键 词：山药总皂苷 抗氧化 DPPH自由基 邻苯三酚超氧阴离子 高效液相色谱法 薯蓣皂苷 原薯蓣皂苷 

分 类 号：R285.5[医药卫生—中药学] R927.2[医药卫生—中医学]