A血型抗原减弱的血清学和分子生物学分析  

Serological and molecular biological analysis of subtypes A degradation

作　　者：黄颖[1] 张瑛[1] 裘晓乐[1] 林甲进[1] HUANG Ying;ZHANG Ying;QIU Xiao-le;LING Jia-jin(Department of Blood Transfusion,the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325027,China)

机构地区：[1]温州医科大学附属第二医院育英儿童医院输血科,浙江温州325027

出　　处：《中国卫生检验杂志》2020年第13期F0002-F0002,F0003,共2页Chinese Journal of Health Laboratory Technology

基　　金：温州市科技计划项目资助课题(Y20140612,Y20190-441);贺林院士工作站科研基金重点项目(18331104)。

摘　　要：目的探讨A血型抗原减弱标本的血清学和分子机制研究。方法采用血清学凝集试管法检测ABO血型,再对标本进行聚合酶链反应和ABO基因6、7外显子及部分内含子的基因测序分析。结果5例标本ABO血型检测正反定型不一致,正定型表现为A抗原减弱,与抗-A1均不凝集,反定型与Bc均4+凝集,与Oc均不凝集。血清学结果A3亚型2例、A2亚型2例、Ael亚型1例,基因测序结果分别为A307/O022例、O02/O02、A102/O01、A102/A101各1例。结论A亚型的基因遗传背景复杂,血清学表现为亚型,基因存在异质性。Objective To study the serological and molecular mechanism of A antigen-attenuated specimens.Methods The ABO blood group was detected by serological agglutination test tube.Then specimens were subjected to polymerase chain reaction and sequence analysis of ABO gene 6,7 exons and some introns.Results The positive stereotypes of 5 specimens showed that the A antigen were all weakened,and were not agglutinated with anti-A1 reagent,but were both 4+agglutinated with Bc,and were not agglutinated with Oc.The results of serological tests were 2 cases of A3 subtype,2 cases of A2 subtype,and 1 case of Ael subtype.The results of gene sequencing were 2 cases of A307/O02,1 case of O02/O02,A102/O01 and A102/A101,Conclusion The genetic background of the subtype A is complex,the serology is subtype,and the gene is heterogeneous.

关 键 词：ABO血型系统 血清学 基因检测 测序 

分 类 号：R446.11[医药卫生—诊断学]