磁固相萃取-高效液相色谱-串联质谱法测定蜂蜜中的克百威  

Determination of Carbofuranin in Honey by Magnetic Solid Phase Extraction Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：平文卉 杨娟[1] 杨清华[1] 史玉坤[1] PING Wenhui;YANG Juan;YANG Qinghua;SHI Yukun(Nantong Center for Disease Control and Prevention,Nantong 226000,China)

机构地区：[1]南通市疾病预防控制中心,江苏南通226000

出　　处：《食品科技》2020年第5期319-322,共4页Food Science and Technology

基　　金：南通市科技计划项目(YYZ17092,MS12017018-6);南通市卫生和计划生育委员会科研课题计划项目(WKZL2018083)。

摘　　要：建立了检测蜂蜜中克百威残留的磁固相萃取-高效液相色谱-串联质谱联用技术方法。蜂蜜用纯水稀释,经室温离子液体负载环糊精磁性纳米材料提取净化后,采用Agilent EC-C18色谱柱(2.7μm,3.0 mm×100 mm)分离,正离子多反应监测模式监测,外标法定量。结果表明:克百威在0.10~80.00μg/L范围内具有良好线性关系,相关系数为0.9959,检出限为0.40μg/kg,富集倍数为10。进行5、10、30μg/L 3种浓度的样品加标试验,回收率为80.3%~83.2%,相对标准偏差为3.1%~4.8%,表明该方法适用于蜂蜜中克百威的检测。To establish a detection method for carbofuran in honey by magnetic solid phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry.Samples were diluted with pure water,and extracted and cleaned up with room temperature ionic liquid loaded cyclodextrin polymer/Fe3O4 nanocomposites,samples were seprarated in Agilent EC-C18 column(2.7μm,3.0 mm×100 mm).The analytes were detected with mass spectrometer in multiple reaction monitoring mode with external standard.The results showed good linearity with the correlation coefficients of 0.9959 in the range of 0.10~80.00μg/L.The limit of detection was 0.40μg/kg and the preconcentration factor of this proposed method was 10.Under 5,10 and 30μg/L spiked levels,the recoveries ranged from 80.3%to 83.2%with the relative standard deviation of 3.1%~4.8%.This method was suitable for the determination of carbofuran in honey.

关 键 词：室温离子液体负载环糊精磁性纳米材料 固相萃取 高效液相色谱-串联质谱法 克百威 蜂蜜 

分 类 号：O657.63[理学—分析化学]