创伤后应激障碍大鼠血清细胞因子与血浆皮质醇水平的关系  

Serum cytokines and plasma cortisol in rats with post traumatic stress disorder horizontal relationship

作　　者：刘杰 李虎年 赵旭 徐清 杨凯 辜伟伟[2] Liu Jie;Li Hunian;Zhao Xu;Xu Qing;Yang Kai;Gu Weiwei(Hubei Medical University Affiliated People’s Hospital Emergency and Critical Care Medicine Center,Shiyan 442000,China;Shiyan Center for Disease Control and Prevention,Shiyan 442000,China)

机构地区：[1]湖北医药学院附属人民医院急危重症中心,十堰442000 [2]十堰市疾病预防控制中心,442000

出　　处：《中华实验外科杂志》2020年第5期887-889,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金面上项目(2015CFC889)。

摘　　要：目的:探讨创伤后应激障碍(PTSD)大鼠血清细胞因子与血浆皮质醇的变化趋势及相关性。方法:将120只雄性Wistar大鼠(十堰市人民医院实验动物中心提供)随机分为对照组(58只)和PTSD组(62只),利用改良的单一连续应激(SPS & S)制作PTSD大鼠模型。采用Morris水迷宫实验和僵立行为实验检测大鼠行为学表现,应用酶联免疫吸附法(ELISA)检测大鼠血清中白细胞介素(IL)-2、IL-4、IL-6、IL-8及血皮质醇含量。组间比较采用 t检验,多因素分析采用Logistic回归分析。 结果:两组大鼠的平均逃避潜伏期(EL)对照组与PTSD组大鼠EL分别为(13.78±1.82) s和(71.16±5.42) s,PTSD组显著高于对照组( t=5.153, P<0.05);两组大鼠僵立行为百分比结果显示,PTSD组大鼠再次放入电击设备箱后(重新面临恐惧情景时),大鼠表现为明显的僵立行为,活动明显减少,主要为刻板的蹲伏行为,仅头部有轻微的转动,背部拱起。对照组大鼠表现为自由运动。对照组大鼠僵立行为百分比为(11.3±3.2)%,PTSD组大鼠为(61.3±7.1)%,两组比较差异有统计学意义( t=27.021, P<0.05);PTSD组与对照组间血清各IL及皮质醇含量比较,创伤后应激障碍组大鼠血清中IL-2、IL-6、IL-8含量显著高于对照组,差异有统计学意义( P<0.01),PTSD组大鼠血皮质醇浓度明显低于对照组,差异有统计学意义( P<0.05)。两组大鼠血清中IL-4含量差异无统计学意义( t=1.112, P>0.05)。 结论:创伤后应激障碍组大鼠血清细胞因子含量的变化呈正相关,与血浆皮质醇浓度呈负相关,IL-2、IL-6、IL-8及血浆皮质醇可能在PTSD的发病机制中起重要作用。Objective To explore the change trend and correlation of serum cytokines and plasma cortisol in post traumatic stress disorder(PTSD)rats.Methods 120 male Wistar rats were randomly divided into control group(58 rats)and PTSD group(62 rats).PTSD rats were made by modified single continuous stress(SPS&S).Morris water maze test and standstill behavior test were used to detect the behavior of rats,and enzyme-linked immunosorbent assay(ELISA)was used to detect interleukin(IL)-2,IL-4,IL-6,IL-8 and cortisol in serum of rats.Results The average El control group and PTSD group of the two groups of rats(escape Latency,EL)were(13.78±1.82)s and(71.16±5.42)s,respectively,which were significantly higher in PTSD group than in control group(t=5.153,P<0.05);the percentage of standstill behavior in PTSD group was significantly higher than that in control group(P<0.05);after the rats in PTSD group were put into the shock equipment box again(when facing the fear situation again),the rats in PTSD group showed obvious standstill behavior,with significantly reduced activity,mainly rigid crouching behavior,only slight rotation of the head and back arch.The rats in the control group showed free movement.The percentage of standstill behavior in the control group was(11.3±3.2)%,that in the PTSD group was(61.3±7.1)%,the difference between the two groups was statistically significant(t=27.021,P<0.05);the levels of IL-2,IL-6 and IL-8 in the serum of the PTSD group were significantly higher than those of the control group,the difference was statistically significant(P<0.01).The serum cortisol concentration of PTSD group was significantly lower than that of the control group.There was no significant difference in serum IL-4 between the two groups(t=1.112,P>0.05).Conclusion The changes of serum cytokines in PTSD rats were positively correlated with the plasma cortisol concentration.IL-2,IL-6,IL-8 and plasma cortisol may play an important role in the pathogenesis of PTSD.

关 键 词：创伤后应激障碍 细胞因子 前炎性细胞因子 抗炎性细胞因子 血浆皮质醇 

分 类 号：R-332[医药卫生] R749.5