蚜虫胁迫对不同抗性玉米品种叶片内抗氧化指标及活性氧代谢的影响  

Effect of Aphid Stress on the Antioxidant Index and Active Oxygen Metabolism in Leaves of Different Resistant Maize Varieties

作　　者：武德功 王俊[2] 杜军利 詹秋文 黄保宏 李文阳 余海兵 WU Degong;WANG Jun;DU Junli;ZHAN Qiuwen;HUANG Baohong;LI Wenyang;YU Haibing(College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China;Institute of Tobacco,Anhui Academy of Agricultural Sciences,Hefei 230001,China)

机构地区：[1]安徽科技学院农学院,安徽凤阳233100 [2]安徽省农业科学院烟草研究所,安徽合肥230001

出　　处：《云南农业大学学报（自然科学版）》2020年第4期608-613,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家重点研发计划(2018YFD0300902);国家自然科学基金青年科学基金项目(31201529);安徽省教育厅重点项目(KJ2019A0813,KJ2020A0065);安徽省农业科学院人才发展专项资金项目(17F0913)。

摘　　要：【目的】明确玉米蚜吸食抗、感玉米品种后对叶片内抗坏血酸(AsA)和谷胱甘肽(GSH)含量、抗坏血酸过氧化物酶(APX)活性及活性氧代谢的影响。【方法】测定被玉米蚜吸食24、48和96 h后感蚜品种蠡玉16和抗蚜品种郑单958叶片中AsA、GSH、O2-、H2O2和OH-的含量以及APX活性。【结果】被玉米蚜刺吸24、48和96 h后,蠡玉16玉米幼苗叶片中的AsA含量与对照相比显著增高(P<0.05);被玉米蚜吸食48和96 h后,郑单958幼苗叶片内的AsA含量显著高于对照(P<0.05);被玉米蚜吸食24 h后,郑单958幼苗叶片中的GSH含量显著低于对照(P<0.05),被玉米蚜吸食48和96 h后,蠡玉16和郑单958幼苗叶片中的GSH含量均显著高于对照(P<0.05);随着吸食时间的增加,蠡玉16和郑单958叶片中的APX活性逐渐降低;被玉米蚜取食24、48和96 h后,蠡玉16幼苗中的H2O2含量均显著高于对照(P<0.05);而被玉米蚜吸食24 h后,郑单958叶片中的H2O2含量与对照差异不显著(P>0.05),但在48和96 h后显著高于对照(P<0.05);被玉米蚜吸食48和96 h后,蠡玉16幼苗叶片中的OH-含量高于对照,而在24、48和96 h后郑单958幼苗叶片中的OH-含量均高于对照;被蚜虫吸食48和96 h后,郑单958幼苗叶片中的O2-含量高于对照,而在24、48和96 h后蠡玉16幼苗叶片中的O2-含量均高于对照。【结论】被玉米蚜吸食后,抗蚜和感蚜品种叶片中的AsA、GSH、APX含量及活性氧代谢均发生相应的变化,对感蚜品种蠡玉16的活性氧影响较大,对抗蚜品种郑单958影响较小。[Purpose]In order to determine the effect of maize aphid pricking stress on the contents of AsA and GSH, APX activity and active oxygen metabolism in the leaves of resistant and susceptible maize varieties.[Method]The contents of AsA,GSH,O2-, H2O2 and OH- and APX activity in the leaves of susceptible maize aphid cultivar Liyu16 and resistant maize aphid cultivar Zhengdan958 were determined at 24, 48 and 96 hours under maize aphid pricking stress.[Result]The content of AsA in the leaves of Liyu16 maize seedlings increased significantly at 24, 48 and 96 h, while that in Zhengdan958 maize seedlings at 48 and 96 h was significantly higher than that in the control(P<0.05), and the contents of GSH in leaves of Zhengdan958 seedlings were significantly lower than that of the control at 24 h(P<0.05). The contents of GSH in the leaves of Liyu16 and Zhengdan958 seedlings were significantly higher than that of the control at 48 and 96 h of pricking(P<0.05);APX activities in the leaves of Liyu16 and Zhengdan958 varieties decreased gradually with the prolongation of aphid pricking time;the contents of H2O2 in the leaves of Liyu16 varieties were significantly higher at 24, 48 and 96 h under maize aphid pricking stress(P<0.05), but at 24 h under maize aphid pricking stress, the contents of H2O2 in the leaves of Zhengdan958 varieties were significantly higher than that of the control(P<0.05). The contents of OH- in the leaves of Liyu16 seedlings were significantly higher than those of the control at 48 h and 96 h of pricking(P<0.05), while the content of OHin leaves of Zhengdan958 were significantly higher at 24, 48 and 96 h under maize aphid pricking stress(P<0.05). The contents of O2- in the leaves of Zhengdan958 seedlings were significantly higher than those of the control at 48 h and 96 h of pricking(P<0.05), while the content of O2- in the leaves of Liyu16 were significantly higher at 24, 48 and 96 h of maize aphid pricking stress(P<0.05).[Conclusion]The above results indicated that the contents of AsA, GSH, APX and the meta

关 键 词：玉米蚜 抗坏血酸过氧化物酶 活性氧代谢 刺吸时间 抗蚜品种 

分 类 号：S513.01[农业科学—作物学]