长链非编码RNA在Kaposi肉瘤组织中的表达  

Expression of long non-coding RNA in Kaposi's sarcoma

作　　者：刘治全 袁虎 普雄明[3] LIU Zhiquan;YUAN Hu;PU Xiongming(Medical College of Shihezi University,Shihezi 832002,China;Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine,Changsha 410006,China;People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)

机构地区：[1]石河子大学医学院,石河子832002 [2]湖南省中医药研究院附属医院,长沙410006 [3]新疆维吾尔自治区人民医院,乌鲁木齐830001

出　　处：《中国麻风皮肤病杂志》2020年第8期467-472,共6页China Journal of Leprosy and Skin Diseases

摘　　要：目的:检测长链非编码RNA(lncRNA)在Kaposi肉瘤组织和瘤旁正常组织中的表达。方法:利用高通量lncRNA芯片技术检测5例Kaposi肉瘤组织及其瘤旁正常组织的lncRNA表达谱,筛选差异表达的lncRNA,并利用实时荧光定量PCR(qRT-PCR)方法进一步验证芯片结果,同时采用生物信息学分析差异lncRNA靶基因KEGG通路注释。结果:与正常组织相比,Kaposi肉瘤组织中共筛选出717个差异表达的lncRNA,其中上调表达408个,下调表达309个;与正常组织相比,lnc-PXDN-3:3和lnc-PERP-10:2在Kaposi肉瘤组织中均表达上调,与芯片结果一致。生物学过程注释集中在Wnt信号通路、泛素化代谢、TGF-beta信号通路、P53信号通路。结论:lncRNA可能与Kaposi肉瘤发病有关。Objective:To detect the differential expression of long non-coding RNA(lncRNA)in Kaposi's sarcoma(KS)tissues and normal adjacent tissues.Methods:The lncRNA expression profiles were detected by high-throughput lncRNA microarray technology in Kaposi's lesions and the adjacent normal tissues in 5 patients.The lncRNAs of differential expression between lesions and adjacent normal tissues were screened,and the results of the microarray were further verified by qRT-PCR.The KEGG pathway annotation of different lncRNA target genes was analyzed by bioinformatics.Results:A total of 717 lncRNAs of differential expression were screened,of which 408 were up-regulated and 309 were down-regulated in Kaposi's sarcoma tissues.The expression of lnc-PXDN-3:3 and lnc-PERP-10:2 in Kaposi's sarcoma tissues was all significantly higher,which was consistent with the chip results.Biological process annotation focuses on Wnt signal pathway,ubiquitin metabolism,TGF-beta signal pathway,p53 signal pathway and so on.Conclusion:lncRNA may be related to Kaposi's sarcoma.

关 键 词：KAPOSI肉瘤 长链非编码RNA 基因芯片 实时荧光定量PCR 

分 类 号：R732.2[医药卫生—肿瘤]