胆维丁乳对急性肺损伤小鼠肺表面活性物质的影响  

Effect of cholecalciterol cholesterol emulsion on pulmonary surfactant in a mouse model of acute lung injury

作　　者：李野 李斌超[1] 张雅琳[1] 刘宁[1] 刘祖望[1] 孔娟[1] LI Ye;LI Binchao;ZHANG Yalin;LIU Ning;LIU Zuwang;KONG Juan(Department of Clinical Nutrition,Shengjing Hospital,China Medical University,Shenyang 110004,China)

机构地区：[1]中国医科大学附属盛京医院临床营养科,沈阳110004

出　　处：《中国医科大学学报》2020年第8期685-689,693,共6页Journal of China Medical University

基　　金：国家自然科学基金(81170065,81570811)。

摘　　要：目的探讨维生素D前体胆维丁乳(CCE)对脂多糖(LPS)造成的急性肺损伤肺表面活性物质的影响。方法将36只雄性BALB/c小鼠随机分为正常对照组、CCE组、LPS组、LPS+CCE组。检测血清中钙磷含量,监测生存曲线,通过计算肺湿/干质量比及组织HE染色,观察肺水肿及肺组织损伤情况,通过Western blotting、实时PCR检测小鼠肺组织维生素D受体(VDR)及肺表面活性物质结合蛋白(SP)-A、SP-B、SP-C、SP-D表达情况。结果与LPS组相比,LPS+CCE组肺水肿及肺组织损伤情况减轻,小鼠的生存时间延长,肺SP的表达增加。结论 CCE能缓解LPS造成的急性肺损伤,可能与其增加了肺表面活性物质的表达有关。Objective To investigate the effect of the vitamin D precursor,cholecalciterol cholesterol emulsion(CCE) on pulmonary surfactant in acute lung injury induced by lipopolysaccharide(LPS). Methods Thirty-six male BALB/c mice were randomly divided into a control group,CCE group,LPS group,and LPS+CCE group. The serum contents of calcium and phosphorus were measured and the survival curve was monitored. Lung edema and lung tissue injury were assessed by calculating the wet-dry weight ratio of the lungs and tissue staining by hematoxylin and eosin. The expression of vitamin D receptor and surfactant proteins(SP-A,SP-B,SP-C and SPD) were detected by real-time PCR and Western blotting,respectively. Results Compared with the results observed in the LPS group,CCE alleviated pulmonary edema and lung tissue damage,prolonged the survival time of mice,and increased the expression of pulmonary surfactant. Conclusion CCE can reduce acute lung injury and this may be related to the increasing expression of pulmonary surfactant.

关 键 词：维生素D 胆维丁乳 脂多糖 急性肺损伤 肺泡表面活性物质 

分 类 号：R151[医药卫生—营养与食品卫生学]