圣约翰草提取物对慢性不可预知性应激致小鼠抑郁模型的影响  

Effects of St. John’s wort extract on a mouse model of chronic unpredictable stress-induced depression

作　　者：傅松年[1] 王江涛[1] 罗福祥 曾贵荣 李治建 马丽 FU Songnian;WANG Jiangtao;LUO Fuxiang;ZENG Guirong;LI Zhijian;MA Li(Psychological Medical Center,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China;Xinjiang Institute of Traditional Uighur Medicine,Laboratory of Toxicology,Urumqi 803349,China;Hunan Key Laboratory of Pharmacodynamics and Safety Evaluation of New Drugs,Changsha 410331,China)

机构地区：[1]新疆医科大学第一附属医院心理医学中心,乌鲁木齐830011 [2]新疆维吾尔自治区维吾尔医药研究所毒理学研究室,乌鲁木齐803349 [3]新药药效与安全性评价湖南省重点实验室,长沙410331

出　　处：《中国医科大学学报》2020年第8期706-710,715,共6页Journal of China Medical University

基　　金：国家重点研发计划中医药现代化研究专项课题(2018YFC1708006-3);国家“重大新药创制”科技重大专项课题(2017ZX09301060005)。

摘　　要：目的观察圣约翰草提取物对慢性不可预知性应激致小鼠抑郁模型的作用及其机制。方法采用慢性不可预知性应激法建立小鼠抑郁模型,将动物随机分为正常对照组、模型对照组、盐酸氟西汀组、圣约翰草提取物低、中、高[0.2 g/(kg·d)、0.4g/(kg·d)、0.8 g/(kg·d)]剂量组,连续给药4周,给药同时对小鼠进行不同条件应激,末次给药后对小鼠进行自主活动、悬尾检测,检测血清5-羟色胺(5-HT)和去甲肾上腺素(NE)含量,取海马组织观察病理学变化。结果圣约翰草提取物低、中、高剂量组均能逆转模型组指标变化(P <0.05或P <0.01),增加抑郁小鼠血清中5-HT、NE含量(P <0.05),减轻海马组织神经元的损伤。且与盐酸氟西汀组作用相当。结论圣约翰草提取物对慢性不可预知性应激致小鼠抑郁模型具有明显的抗抑郁作用,其作用可能是通过调节神经递质5-HT和NE含量,减轻海马组织神经元损伤。Objective To observe the effect of St. John’s wort extract on a mouse model of chronic unpredictable stress-induced depression and examine its mechanism. Methods The chronic unpredictable stress method was adopted for four weeks to establish the mice model of depression. Test animals were randomly divided into normal control,model control,fluoxetine hydrochloride,and St. John’s wort extract low,medium and high(0.2 g/kg,0.4 g/kg,0.8 g/kg,respectively) dose groups. The sugar water preference of the mice was determined. To induce stress,different conditions after the last dose of autonomous activities were employed were four weeks,and a tail suspension test was performed. Blood was taken to detect the serum 5-HT and NE levels,and the pathological changes in the hippocampus were observed. Results The St. John’s wort extract low,medium,and high dose groups could reverse the above changes(P < 0.05 or P < 0.01),had increased serum 5-HT and NE levels(P < 0.05),and reduced neuronal damage in the hippocampal tissue region. The effects observed in the fluoxetine hydrochloride group were similar. Conclusion St. John’s wort extract had a noticeable anti-inhibitory effect on the mouse model of depression caused by chronic unpredictable stress. Its effect may be related to the alleviation of neuronal damage in the hippocampal tissue by regulating the levels of the neurotransmitters,5-HT and NE.

关 键 词：圣约翰草 抑郁 应激 自主活动 悬尾 

分 类 号：R749.92[医药卫生—神经病学与精神病学]