LSD1和PDPN在舌鳞状细胞癌中的表达及对预后的影响  

Expressions of LSD1 and PDPN in tongue squamous cell carcinoma and their effects on prognosis

作　　者：王坤龙 张洋[1] 宿伟鹏[1] 刘攀[1] 赵化荣[1] Wang Kunlong;Zhang Yang;Su Weipeng;Liu Pan;Zhao Huarong(Cancer Center,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学第一附属医院肿瘤中心,乌鲁木齐830000

出　　处：《国际肿瘤学杂志》2020年第5期264-271,共8页Journal of International Oncology

基　　金：新疆维吾尔自治区自然科学基金(2016D01C263)。

摘　　要：目的探讨组蛋白赖氨酸特异性去甲基化酶1(LSD1)、平足蛋白(PDPN)在舌鳞状细胞癌组织中的表达及其与患者临床病理特征和预后的关系。方法选取新疆医科大学第一附属医院2011年1月至2016年1月67例舌鳞状细胞癌及相应癌旁正常组织标本,采用免疫组织化学法检测癌组织及癌旁组织中LSD1、PDPN的表达情况,并长期随访患者,分析LSD1和PDPN的表达与患者临床病理特征和预后的相互关系。结果LSD1和PDPN在舌鳞状细胞癌组织中的表达强度高于癌旁组织,差异具有统计学意义(Z=6.089,P<0.001;Z=5.781,P<0.001)。LSD1和PDPN的表达强度在不同临床分期(χ2=11.487,P=0.001;χ2=8.111,P=0.004)、淋巴结转移(χ2=4.772,P=0.029;χ2=6.206,P=0.013)、肿瘤大小(χ2=5.985,P=0.014;χ2=4.247,P=0.039)患者中差异均具有统计学意义。LSD1的表达强度在不同分化程度患者中差异也具有统计学意义(χ2=6.660,P=0.010)。单因素分析中,LSD1表达强度与患者无进展生存期(PFS)及总生存期(OS)呈负相关(χ2=18.930,P<0.001;χ2=16.257,P<0.001),PDPN表达强度与患者PFS及OS呈负相关(χ2=31.720,P<0.001;χ2=18.390,P<0.001),肿瘤大小与患者PFS和OS呈负相关(χ2=5.326,P=0.021;χ2=8.843,P=0.003);术后放疗、临床分期分别与患者的OS呈正、负相关(χ2=4.197,P=0.040;χ2=6.355,P=0.012)。多因素分析中,LSD1是患者PFS和OS的的独立危险因素(HR=5.743,95%CI为1.012~32.579,P=0.048;HR=17.759,95%CI为2.303~136.916,P=0.006),PDPN是患者PFS的独立危险因素(HR=4.380,95%CI为1.258~15.254,P=0.020),术后放疗是患者PFS及OS的保护因素(HR=0.374,95%CI为0.157~0.895,P=0.027;HR=0.218,95%CI为0.091~0.521,P=0.001),临床分期是患者OS的独立危险因素(HR=2.637,95%CI为1.107~6.280,P=0.029)。在舌鳞状细胞癌组织中,LSD1与PDPN的表达呈正相关(rs=0.655,P<0.001)。结论LSD1和PDPN在舌鳞状细胞癌组织中的表达均高于癌旁组织;LSD1是患者PFS和OS的独立危险因素,PDPN是患者PFS的独立危险因素Objective To investigate the expressions of lysine specific demethylase 1(LSD1)and podoplanin(PDPN)in tongue squamous cell carcinoma and the correlation between LSD1 or PDPN and clinicopathological characteristics or prognosis.Methods A total of 67 cases of tongue squamous cell carcinoma and corresponding paracancerous normal tissues in the First Affiliated Hospital of Xinjiang Medical University from January 2011 to January 2016 were selected.The expressions of LSD1 and PDPN in cancer and paracancerous tissues were detected by immunohistochemical method,and the patients were followed up for a long time to analyze the correlation between the expression of LSD1 or PDPN and clinicopathological characteristics or prognosis.Results The expressions of LSD1 and PDPN in tongue squamous cell carcinoma tissues were higher than those in paracancerous tissues,and the differences were statistically significant(Z=6.089,P<0.001;Z=5.781,P<0.001).The expression intensities of LSD1 and PDPN were significantly different in patients with different clinical stage(χ2=11.487,P=0.001;χ2=8.111,P=0.004),lymph node metastasis(χ2=4.772,P=0.029;χ2=6.206,P=0.013)and tumor size(χ2=5.985,P=0.014;χ2=4.247,P=0.039).The expression intensity of LSD1 was also significantly different in patients with different degrees of differentiation(χ2=6.660,P=0.010).In univariate analysis,LSD1 expression intensity was negatively correlated with progression-free survival(PFS)and overall survival(OS)(χ2=18.930,P<0.001;χ2=16.257,P<0.001),PDPN expression intensity was negatively correlated with PFS and OS(χ2=31.720,P<0.001;χ2=18.390,P<0.001),and tumor size was negatively correlated with PFS and OS(χ2=5.326,P=0.021;χ2=8.843,P=0.003).Postoperative radiotherapy and clinical stage were positively and negatively correlated with OS respectively(χ2=4.197,P=0.040;χ2=6.355,P=0.012).In multivariate analysis,LSD1 was an independent risk factor for PFS and OS(HR=5.743,95%CI:1.012-32.579,P=0.048;HR=17.759,95%CI:2.303-136.916,P=0.006),PDPN was an independent risk

关 键 词：舌肿瘤 预后 组蛋白赖氨酸特异性去甲基化酶1 平足蛋白 

分 类 号：R739.86[医药卫生—肿瘤]