中性粒细胞外泌体诱导骨髓间充质干细胞分泌成血管相关因子的研究  

The effects of PMN-derived exosomes on the angiogenesis-related genes of hBMSCs

作　　者：谢纪荣 黄鑫 牟亚群 赵路 黎哲昊 蔡卜磊 高士军[1] XIE Jirong;HUANG Xin;MOU Yaqun;ZHAO Lu;LI Zhehao;CAI Bolei;GAO Shijun(Department of Prosthodontics,School of Stomatology,Jiamusi University,China,154003;Schoo of Stomatology of Qingdao University,Department of Orthodontics,Qingdao Stomatological Hospital;State Key Laboratory of Military Stoma tology&National Clinical Research Center for Oral Diseases&Shaanxci Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,School of Stomatology,The Fourth Military Medical University,Xi’an)

机构地区：[1]佳木斯大学口腔医学院,佳木斯大学口腔医院修复科,154003 [2]青岛大学医学部口腔医学院,青岛市口腔医院中心实验室,青岛市口腔医院正畸科 [3]军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,第四军医大学口腔医院颌面外科

出　　处：《实用口腔医学杂志》2020年第4期562-566,共5页Journal of Practical Stomatology

基　　金：军事口腔医学国家重点实验室自主研究课题(编号:2018-ZB04)。

摘　　要：目的:探索中性粒细胞来源外泌体(PMN-Exo)体外诱导人骨髓间充质干细胞(hBMSCs)分泌促血管生成的相关因子。方法:免疫磁珠法提取并超离法分离PMN-Exo,对PMN-Exo进行鉴定及内吞实验;细胞实验分为PBS组和PMN-Exo组,EdU法检测细胞增殖活性,划痕实验检测细胞迁移能力,并对PMN-Exo诱导hBMSCs分泌成血管相关因子的能力进行检测。结果:成功分离鉴定PMN-Exo,并证实PMN可有效进入hBMSCs。PMN-Exo组与PBS组比较,细胞增殖活性、迁移能力明显增强,并大量合成VEGF和PDGF。结论:PMN-Exo可显著促进hBMSCs的增殖和迁移能力,促进其成血管相关因子的表达。Objective: To study the effects of PMN-derived exosomes(PMN-Exo) on the promotion of angiogenesis-related factors of hBMSCs in vitro. Methods: PMNs from the mouse marrow were extracted by magnetic activated cell sorting. PMN-Exo obtained by supercentrifugation and PMN-Exo internalization into hBMSCs were identified. hBMSCs were treated with PMN-Exo(PMN-Exo group) and PBS(PBS group) respectively. The promotion effect of PMN-Exo on the expression of VEGF and PDGF of hBMSCs was studied. Cell proliferation of hBMSCs were detected by EdU staining. Cell migration was assessed by scratch test. The mRNA expression of VEGF and PDGF of hBMSCs was analyzed by QPCR and WB. Results: PMN-Exo was isolated and conformed by the identification. Compared with PBS group, PMN-Exo group showed stronger proliferation, cell migration and higher expression of VEGF and PDGF. Conclusion: PMN-Exo can promote the proliferation, migration and the expression of angiogenesis-related factors of hBMSCs.

关 键 词：中性粒细胞(PMN) 外泌体 HBMSCS 成血管相关因子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]