积雪草酸对人牙周膜细胞增殖、迁移和成骨分化的影响  

The effects of asiatic acid on the proliferation,migration and osteogenic differentiation of human perio-dontal ligament cells

作　　者：郝春波[1] 邓伟[1] 巩蕾[1] 谭毅 王周凯欣 HAO Chunbo;DENG Wei;GONG Lei;TAN Yi;WANG Zhoukaixin(Department of Sto-matology,Hainan General Hospital,Hainan Affiliated Hospital of Hainan Medical Uniwersity,China,Haihou 570311;School of Management,Hainan Medical University,Haikou)

机构地区：[1]海南省人民医院·海南医学院附属海南医院口腔科,海口570311 [2]海南医学院管理学院

出　　处：《实用口腔医学杂志》2020年第4期666-670,共5页Journal of Practical Stomatology

基　　金：海南省重点研发计划项目(编号:ZDYF2019216);海南省高等学校科学研究重点项目(编号:Hnky2019ZD-22);海南省自然科学基金(编号:817320)。

摘　　要：目的:探讨不同浓度积雪草酸(AA)对人牙周膜细胞(hPDLCs)增殖、迁移和成骨分化能力的影响。方法:以浓度为25、50、100μg/ml的AA处理体外培养的hPDLCs,分别培养1~7d,CCK8法、Transwell小室迁移实验检测各组细胞增殖和迁移能力;ALP和茜素红染色、实时荧光PCR、蛋白免疫印迹检测各组细胞的矿化能力。结果:不同浓度的积雪草酸对人牙周膜细胞的增殖和迁移能力无明显影响。在矿化诱导的条件下,50μg/ml积雪草酸处理的细胞成骨分化能力显著增强。结论:25~100μg/ml的AA不影响hPDLCs的增殖和迁移能力,但50μg/ml时可促进其成骨分化。Objective: To evaluate the effects of asiatic acid(AA) on the proliferation, migration and osteogenic differentiation of human periodontal ligament cells(hPDLCs). Methods: hPDLCs were in vitro cultured and treated with AA a 25, 50 and 100 μg/ml for 1-7 d respectively. The proliferation and migration capacity of hPDLCs were detected by CCK8 assay and transwell migration assay, respectively. The mineralization capacity was tested by ALP staining and Alizarin red staining, the osteogenic differentiation related gene and protein expression was examined by qRT-PCR and Western blot. Results: AA at 25-100 μg/ml had no effects on the proliferation and migration of hPDLCs. However, the concentration of 50 μg/ml AA promoted the osteogenic capacity of hPDLCs under osteogenic induction. Conclusion: AA at 25-100 μg/ml may not effect the proliferation and migration capacities of PDLCs. AA at 50 μg/ml may promote the osteogenic differentiation of hPDLCs under osteogenic induction.

关 键 词：积雪草酸(AA) 人牙周膜细胞(hPDLCs) 成骨分化 增殖 迁移 

分 类 号：R781.4[医药卫生—口腔医学]