虾青素对1型糖尿病大鼠视网膜病变的保护作用  

Protective effect of astaxanthin on the retinopathy of type 1 diabetic rats

作　　者：杨明 赵通[2] 邓婷婷[2] 潘琳[2] 王志军[2] Yang Ming;Zhao Tong;Deng Tingting;Pan Lin;Wang Zhijun(Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University,Beijing Ophthalmology﹠Visual Sciences Key Lab,Beijing 100730,China;Department of Ophthalmology,China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科研究所,北京市眼科学与视觉科学重点实验室,北京100730 [2]中日友好医院眼科,北京100029

出　　处：《中华实验眼科杂志》2020年第7期589-596,共8页Chinese Journal Of Experimental Ophthalmology

摘　　要：目的:观察虾青素对1型糖尿病大鼠视网膜病变的保护作用及相关机制。方法:36只SPF雄性大鼠应用腹腔内一次性注射质量分数1%链脲佐菌素(STZ)方法制备大鼠糖尿病模型,采用随机数字表法分为糖尿病组、低剂量虾青素组和高剂量虾青素组,每组9只。低剂量虾青素组和高剂量虾青素组每日分别给予20 mg/kg和100 mg/kg虾青素灌胃,糖尿病组同法给予等量橄榄油。另设对照组大鼠12只,同法给予等量枸橼酸钠缓冲液。每2周测量大鼠血糖和体质量。24周后,过量水合氯醛麻醉法处死大鼠,眼球称质量标记。经胰蛋白酶消化制作视网膜毛细血管铺片,每张铺片任意选取5个视野计算周细胞与无细胞性毛细血管的数量。通过免疫组织化学、实时PCR和Western blot法测定各组大鼠视网膜组织中白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)和凋亡蛋白家族caspase-3的相对表达量。结果:低剂量虾青素组和高剂量虾青素组大鼠体质量均高于糖尿病组,差异均有统计学意义(均P<0.05)。低剂量虾青素组和高剂量虾青素组大鼠血糖较糖尿病组降低,差异均统计学意义(均P<0.05)。高倍镜下,对照组毛细血管周细胞核小,呈圆形或三角形;糖尿病组视网膜毛细血管局部扩张、狭窄,扭结成襻。低倍镜下,糖尿病组可见视网膜动静脉主干及分支迂曲,毛细血管网紊乱;高剂量虾青素组血管走向较迂曲,毛细血管局部扩张、狭窄程度有所减轻。各组周细胞计数分别为466.4±23.2、207.3±31.7、298.1±27.1和312.2±19.5,总体比较差异有统计学意义(F=34.420,P=0.047),其中低剂量虾青素组、高剂量虾青素组周细胞计数高于糖尿病组,差异均有统计学意义(均P<0.05)。各组无细胞毛细血管计数分别为5.2±2.3、32.9±12.7、14.5±9.1和16.5±3.5,总体比较差异有统计学意义(F=47.340,P=0.021),其中低剂量虾青素组、高剂量虾青素组无细胞性毛细血管数�Objective To investigate the protective effect of astaxanthin on the retinopathy in rats with type 1 diabetes and related mechanism.Methods Thirty-six male SPF rats received intraperitoneal injection of 1%streptozotocin(STZ)to prepare type 1 diabetes model.The rats were randomly assigned to the diabetes group,low dose astaxanthin group and high dose astaxanthin group by a random number table.The rats in the low dose astaxanthin group and high dose astaxanthin group received respectively astaxanthin 20 mg/kg and 100 mg/kg by gavage everyday.The rats in the diabetes group received an equal volume of olive oil.Twelve rats received an equal volume of sodium citrate as the control group.Blood glucose and body mass were measured every 2 weeks.After 24 weeks,the retina was digested to make retinal capillary network preparation.The number of pericytes and acelluar strands was compared among the different experimental groups.The relative expressions of anti-inflammatory cytokines in retinal tissue were detected by immunohistochemistry,real-time PCR and Western blot.The use and care of the animals complied with Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results Body mass of the rats in the low dose astaxanthin group and high dose astaxanthin group was significantly higher than that in the diabetes group(both at P<0.05).Blood glucose levels in the low dose astaxanthin group and high dose astaxanthin group were significantly lower than that in the diabetes group(both at P<0.05).In the control group,main artery was round,uniform,and strongly stained.The vein was lightly stained and had a large diameter.In the diabetes group,retinal arteriovenous trunk and branches appeared tortuous at a low magnification,with capillary network disorder.The morphology of the capillaries showed pathology in the low dose astaxanthin group and high dose astaxanthin group,but the vascular tortuosity,dilatation and stenosis were reduced in comparison with the diabetes grou

关 键 词：虾青素 糖尿病 视网膜 周细胞 抗凋亡 抗炎 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]