快速眼动期睡眠剥夺诱导大鼠焦虑相关自我修饰行为及血清5-HT浓度的研究  

Anxiety-related self-modifying behavior and serum 5-HT levels in rats with REM sleep deprivation

作　　者：刘文娟[1] 毛洪京[1] 余正和[1] 刘义[1] 杨莉莉 韦又丹 邵琼琰[2] LIU Wenjuan;MAO Hongjing;YU Zhenghe;LIU Yi;YANG Lili;WEI Youdan;SHAO Qiongyan(Department of Psychosomatic Diseases,Hangzhou Seventh People’s Hospital,Hangzhou 310013,China)

机构地区：[1]杭州市第七人民医院心身一科,310013 [2]浙江中医药大学

出　　处：《浙江医学》2020年第14期1480-1484,共5页Zhejiang Medical Journal

基　　金：浙江省中医药科学研究基金项目(20192A100);杭州市卫生科技计划项目(2016A47)。

摘　　要：目的观察快速眼动期(REM)睡眠剥夺诱导大鼠焦虑相关自我修饰行为指标以及与血清5-羟色胺(5-HT)浓度的相关性。方法将24只大鼠随机分为睡眠剥夺组和对照组,每组12只。对照组大鼠常规饲养,不予干扰睡眠;睡眠剥夺组采用改良的多平台水环境法,通过72 h REM睡眠剥夺诱导大鼠焦虑样行为。造模前后两组均进行旷场(OF)试验,造模后两组通过高架O迷宫(EOM)试验和自我修饰行为分析进行焦虑的量化评定,并检测血清5-HT浓度。结果造模前,睡眠剥夺组水平和垂直分数与对照组比较差异均无统计学意义(均P>0.05);造模后睡眠剥夺组水平和垂直分数均明显低于对照组(均P<0.01);睡眠剥夺组进入闭合臂次数多于对照组,进入开放臂次数的百分比低于对照组(均P<0.05);睡眠剥夺组错误的转换次数(IT)、中断修饰的次数、错误的转换百分比(IT%)和中断梳理的百分比均较对照组为高(均P<0.01);对照组血清5-HT浓度高于睡眠剥夺组,睡眠剥夺组IT%与5-HT浓度之间存在负向相关性(P<0.05)。结论REM睡眠剥夺诱导了焦虑样行为,且其中自我修饰行为参数与血清5-HT浓度存在一定相关性。Objective To investigate the effect of rapid eye movement(REM)sleep deprivation(SD)on anxiety-related self-modifying behavior and serum 5-HT levels in rats.Methods Twenty four rats were randomly divided into control group and SD group with 12 rats in each group.The anxiety-like behavior rat model was induced by 72 h sleep deprivation with the modified multi-platform water environment method.The OF and EOM tests were performed before and after modeling and Grooming analysis algorithm was used to quantify the anxiety-like behaviors induced by REM sleep deprivation;the serum 5-HT levels were detected.Results Before modeling,there were no statistically significant differences in horizontal and vertical scores between the SD group and the control group(all P>0.05).After modeling,the horizontal and vertical scores of OF experiment in SD group were significantly lower than in control group(both P<0.01).The mean number of CE in SD group was significantly more than that of control group(P<0.05).The percentage of open area entry(OE%)in SD group was significantly lower than that in control group(P<0.05).The grooming analysis algorithm found that SD group had significantly changed,IT,IB,IT%and IB%were significantly higher than those in control group(all P<0.01).The level of 5-HT in control group was significantly higher than that in the SD group,and IT%was negatively correlated with 5-HT in SD group(P<0.05).Conclusion After 72 h of sleep deprivation,the behavioral parameters in rats were significant changed.The results show that REM sleep deprivation can induce anxiety behavior,which is associated with serum 5-HT levels in SD rats.

关 键 词：睡眠剥夺 焦虑 修饰 5-羟色胺 

分 类 号：R740[医药卫生—神经病学与精神病学] R749.72[医药卫生—临床医学]