幽门螺杆菌感染对慢性浅表性胃炎患者胃内菌群的影响  

Effects of Helicobacter pylori infection on gastric flora in patients with chronic superficial gastritis

作　　者：刘颖[1] 许文侠 黄彩群 王华斌[1] LIU Ying;XU Wenxia;HUANG Caiqun;WANG Huabin(Central Laboratory,Jinhua Central Hospital,Jinhua 321000,China)

机构地区：[1]金华市中心医院(浙江大学附属金华医院)中心实验室,321000

出　　处：《浙江医学》2020年第14期1496-1499,1503,I0004,I0005,共7页Zhejiang Medical Journal

基　　金：金华市科技计划项目(2017-3-015)。

摘　　要：目的探讨幽门螺杆菌(Hp)感染对慢性胃炎患者胃内菌群丰度和多样性的影响。方法选取2017年10月至2018年6月金华市中心医院收治行胃镜检查的慢性浅表性胃炎患者38例,胃黏膜组织病理学检查和细菌培养结果中任一项为Hp阳性,归为Hp阳性组(Hp-P组,14例);两者均为阴性,归为Hp阴性组(Hp-N组,24例)。将胃黏膜组织研磨后提取基因组DNA,对16S r RNA基因V3~V4可变区进行基因扩增,构建基因文库,基于Illumina Miseq测序平台进行测序。测序数据经生物信息处理后,将得到的有效序列按相似度0.97进行分类单位聚类。比较Hp-P组和Hp-N组菌群的丰度和多样性,以及物种相似性。结果Hp-P组患者胃内菌群的丰度和多样性均明显低于Hp-N组;Hp-P组和Hp-N组物种相似性比较,两组组内菌群结构相似性较高,而两组之间菌群结构存在明显差异。Hp-P组的优势菌群分别为Helicobacter(89.1%)、Prevotella(1.2%)、Methylobacterium(1.1%)、Sphingomonas(1.1%);Hp-N组优势菌群分别为Prevotella(14.5%)、Streptococcus(10.1%)、Neisseria(7.1%)、Haemophilus(6.5%)、Sphingomonas(4.6%)、Veillonella(4.6%)、Methylobacterium(4.6%)。Hp与胃内其他菌群呈负作用关系。结论Hp感染会明显降低慢性浅表性胃炎患者胃内菌群的丰度和多样性。Objective To investigate the influence of Helicobacter pylori(Hp)on the gastric microbiota in chronic superficial gastritis patients.Methods Gastric mucosa tissue samples were obtained from 38 patients with pathologically confirmed superficial gastritis.The patients with either pathology or bacterial culture positive were classified as Hp positive group(Hp-P)and those with both negative were classified as Hp negative group(Hp-N).Genome DNA was extracted from gastric mucosa tissue,then the variable regions of 16 S r RNA V3~V4 DNA were amplified and the fragment gene library was constructed.The raw sequences data were obtained that were based on the Illumina Miseq platform.Raw data were processed by bioinformatics and effective sequences were obtained.The sequences were clustered into the OUTs with 97%consistency.The bacterial abundance,diversity and similarity were compared between Hp-P and Hp-N groups.Results The bacterial abundance and diversity in Hp-P group were significant lower than those in Hp-N group.The intragroup similarity of microbial community was high in both groups,but the intergroup difference was significant.The predominant species in Hp-P group were Helicobacter(89.1%),Prevotella(1.2%),Methylobacterium(1.1%),Sphingomonas(1.1%);while the predominant species in Hp-N group were Prevotella(14.5%),Streptococcus(10.1%),Neisseria(7.1%),Haemophilus(6.5%),Sphingomonas(4.6%),Veillonella(4.6%),Methylobacterium(4.6%).It was a negative relationship between Hp and other bacterial in the stomach.Conclusion The gastric microbial abundance and diversity are significant lower in chronic gastritis patients due to Hp infection.

关 键 词：幽门螺杆菌 胃内菌群 慢性浅表性胃炎 

分 类 号：R573.31[医药卫生—消化系统]