MiR-29b expression is altered in crescent formation of HSPN and accelerates AngⅡ-induced mesangial cell activation  

作　　者：Shan Cheng Chun-Hua Zhu Ai-Hua Zhang Song-Ming Huang 

机构地区：[1]Department of Infectious Disease,Children's Hospital of Nanjing Medical University,72 Guangzhou Road,Nanjing 210008,China [2]Department of Nephrology,Children's Hospital of Nanjing Medical University,72 Guangzhou Road,Nanjing 210008,China

出　　处：《World Journal of Pediatrics》2020年第2期201-212,共12页世界儿科杂志（英文版）

基　　金：This study was supported by Clinical Medicine Science and Technology Project of Jiangsu Province (BL2014007).

摘　　要：Background MicroRNA-29b(miR-29b)has been suggested to possess pro-inflammatory activity,which can partially be explained by the repression of tumor necrosis factor alpha protein three antibody(TNFAIP3).Meanwhile,it also promotes thyroid cell proliferation via Smad signaling pathways.The present study aimed to elucidate the role of miR-29b in Henoch Sch(o)nlein purpura nephritis(HSPN)and its underlying molecular mechanism in angiotensinⅡ(AngⅡ)-induced human glomerular mesangial cell(HGMC)activation.Methods We evaluated miR-29b expression in 35 HSPN renal tissues based on crescent formation,glomerular sclerosis,interstitial fibrosis,thrombosis formation and capillary loop necrosis.Meanwhile,HGMCs were cultured,treated with AngⅡand then transfected with LV-hsa-miR-29b-1 to induce miR-29b overexpression or LV-hsa-miR-29b-3p-inhibition to inhibit miR-29b expression.Finally,we examined the effects of miR-29b on cell proliferation and release of inflammatory mediators.Results We observed that miR-29b expression was significantly higher in the crescent group than in the no crescent group.MiR-29b overexpression induced the release of intercellular adhesion molecule-1,interleukin-1β(IL-1β),IL-6,IL-8,the increase of CyclinA2,CyclinD 1,and cell proliferation.It also could inhibit the expressions of TNFAIP3 and NF-kappa-B-repressing factor(NKRF).Correspondingly,miR-29b inhibition produced the opposite effects and increased the expression of TNFAIP3 and NKRF.Conclusion MiR-29b expression is altered in crescent formation of HSPN and accelerates AngⅡ-induced mesangial cell proliferation and release of inflammatory mediators.

关 键 词：Cell proliferation CRESCENT INFLAMMATION MiR-29b 

分 类 号：R72[医药卫生—儿科]