NF-κB在幽门螺杆菌感染介导的胃癌发生发展中的作用  

Role of NF-κB in the Development of Gastric Cancer Mediated by Helicobacter Pylori Infection

作　　者：杨柳青[1] 铁茹[2] 陈建婷[1] 王军艳[1] 蒋承志[1] 牟花妮 张鸣[1] YANG Liu-qing;TIE Ru;CHEN Jian-ting;WANG Jun-yan;JIANG Cheng-zhi;MU Hua-ni;ZHANG Ming(The Second Affiliated Hospital of Xi'an Medical College,1 Department of Gastroenterology;Department of Scientific Research,Xi'an,Shaanxi,710038,China)

机构地区：[1]西安医学院第二附属医院消化科,陕西西安710038 [2]西安医学院第二附属医院科研科,陕西西安710038

出　　处：《现代生物医学进展》2020年第11期2106-2109,共4页Progress in Modern Biomedicine

基　　金：陕西省教育厅自然科学基金项目(12Jk1032)。

摘　　要：目的:探讨核因子-κB(nuclear factor-κB,NF-κB)在幽门螺杆菌感染介导的胃癌发生发展中的作用。方法:选择2016年3月至2019年3月在本院诊治的胃部疾病患者110例,采用qPCR检测NF-κB相对表达情况,采用免疫印记法检测幽门螺杆菌(Helicobacter pylori,Hp)感染情况并进行相关性分析。结果:在110例患者中,病理诊断为胃癌9例(胃癌组)和良性胃部疾病101例(良性组,其中浅表性胃炎52例、萎缩性胃炎26例、不典型增生23例)。胃癌组的幽门螺杆菌感染率为88.9%,显著高于良性组的10.9%(P<0.05)。胃癌组的NF-κB表达阳性率为77.8%,显著高于良性组的14.9%(P<0.05)。在110例患者中,直线相关性分析显示幽门螺杆菌感染、NF-κB表达阳性与胃癌有显著正相关性(P<0.05)。受试者工作特征曲线(receiver operating characteristic curve,ROC)显示幽门螺杆菌感染、NF-κB表达阳性鉴别诊断胃癌的曲线下面积分别为0.669和0.713。结论:NF-κB在胃癌中呈现高表达状况,也多伴随有幽门螺杆菌感染,两者存在显著相关性,共同介导胃癌的发生发展。Objective:To investigate the role of nuclear factor-κB(NF-κB)in the development of gastric cancer mediated by Helicobacter pylori infection.Methods:From March 2016 to March 2019,a total of 110 patients with gastric diseases diagnosed and treated were enrolled.qPCR were used to detect the relative expression of NF-κB,and Helicobacter pylori(Hp)infection were detected by immunoblotting.And carry out correlation analysis.Results:In the 110 patients,there were 9 patients of gastric cancer(gastric cancer group)and 101 patients of benign gastric disease(benign group that included 52 patients of superficial gastritis,26 patients of atrophic gastritis,and 23 patients of dysplasia).The Helicobacter pylori infection rates were 88.9%in the gastric cancer group,which were significantly higher than that in the benign group(10.9%)(P<0.05).The positive rates of NF-κB expression in gastric cancer group were 77.8%,which were significantly higher than that in benign group(14.9%)(P<0.05).In the 110 patients,linear correlation analysis showed that Helicobacter pylori infection and NF-κB expression were positively correlated with gastric cancer(P<0.05).The receiver operating characteristic curve(ROC)showed that the area under the curve of Helicobacter pylori infection and NF-κB positive differential diagnosis of gastric cancer were 0.669 and 0.713,respectively.Conclusion:NF-κB are highly expressed in gastric cancer,and it are accompanied by Helicobacter pylori infection.There are significant correlation between them which together mediate the development of gastric cancer.

关 键 词：核因子-ΚB 幽门螺杆菌 胃癌 相关性 胃炎 

分 类 号：R735.2[医药卫生—肿瘤] R573.3[医药卫生—临床医学]