小麦‘Fielder’ ω-醇溶蛋白基因及启动子的克隆与序列分析  

Cloning and sequence analysis of the coding region and promoter of ω-gliadin genes from wheat ’Fielder’(Triticum aestivum L.)

作　　者：郭丹丹 李保云 GUO Dandan;LI Baoyun(College of Agronomy and Biotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis and Utilization of Ministry of Education,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学农学院/北京市作物遗传改良重点实验室/教育部作物杂种优势研究与利用重点实验室,北京100193

出　　处：《中国农业大学学报》2020年第7期1-9,共9页Journal of China Agricultural University

基　　金：转基因生物新品种培育重大专项(2016ZX08002003);国家自然科学基金(31371607)。

摘　　要：为研究六倍体小麦中ω-醇溶蛋白基因簇的启动子差异,以普通小麦品种‘Fielder’为试验材料,利用同源克隆的方法,克隆ω-醇溶蛋白基因的编码区和启动子序列,并进行生物信息学相关分析。结果表明:1)共克隆得到11条不同的基因序列(MN441496~MN441506),其中6条(MN441496~MN441497和MN441503~MN441506)编码ARE/Q型ω-醇溶蛋白,5条(MN441498~MN441502)编码SRL型ω-醇溶蛋白。2)ARE/Q型ω-醇溶蛋白基因编码区长度范围在972~1158bp,SRL型ω-醇溶蛋白基因编码区长度范围在1303~1419bp,这种差异主要由中间重复区的Indel类型和数量不同造成。3)ARE/Q型ω-醇溶蛋白基因启动子在-300bp含有1个典型的endosperm box,而SRL型ω-醇溶蛋白基因启动子在-300和-600bp处各含有1个endosperm box。这些ω-醇溶蛋白基因启动子上的差异,可能引起表达水平的差异。To understand the promoter differences ofω-gliadin gene cluster in hexaploid wheat,the coding region along with the promoter sequences ofω-gliadin in hexaploid common wheat cultivar’Fielder’were obtained by homologous cloning.Bioinformatics analysis was conducted.The results showed that:1)A total of 11 differentω-gliadin gene sequences were acquired,and six of which belonged to the ARE/Q type genes,the rest five were SRL type genes.2)The coding region lengths were varied from 972 to 1158 bp among the six ARE/Q type genes and from 1303 to 1419 bp among the five SRL type genes,which were the results of differences in types and quantities of Indels in the center repeat region.3)There located a conserved typical endosperm box at-300 bp upstream of ARE/Q typeω-gliadin genes.Nevertheless,the endosperm box appeared at-300 and-600 bp upstream of SRL typeω-gliadin genes,respectively.The differences in the promoter ofω-gliadin gene cluster might influence the expression levels of differentω-gliadin genes.

关 键 词：小麦 A-PAGE 醇溶蛋白 启动子 endosperm box 

分 类 号：Q946.2[生物学—植物学]