地黄不同炮制品薄层色谱鉴别方法的建立  

Establishment of Thin Layer Chromatography Identification Method for Different Processed Products of Rehmanniae Radix

作　　者：张颖 孙菲菲 杨佳宁 张江山 张振凌[1] ZHANG Ying;SUN Fei-fei;YANG Jia-ning;ZHANG Jiang-shan;ZHANG Zhen-ling(Henan Engineering Research Center for Characteristic Processing Technology of Traditional Chinese Medicine,College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450008,China)

机构地区：[1]河南中医药大学药学院,河南省中药特色炮制技术工程研究中心,郑州450008

出　　处：《中国实验方剂学杂志》2020年第15期142-149,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中药标准化项目(ZYBZH-Y-HEN-17,ZYBZH-Y-HEN-18);国家重点研发计划项目(2017YFC1702800,2018YFC1707202);河南中医药大学研究生科研创新基金项目(YJS 2018B11)。

摘　　要：目的:建立地黄不同炮制品的薄层色谱鉴别方法。方法:以D-果糖、梓醇、蔗糖、棉子糖、水苏糖、蜜二糖及甘露三糖为对照品,考察提取溶剂(水,20%甲醇,50%甲醇,80%甲醇),展开剂(正丁醇-甲醇-三氯甲烷-冰乙酸-水,乙酸乙酯-吡啶-冰乙酸-水,正丁醇-冰乙酸-水),显色剂(苯胺-二苯胺-磷酸溶液,茚三酮溶液),点样量(2,4,6μL),检视条件(日光,日光底部灯,365 nm和254 nm)对薄层色谱分析的影响,确定生地黄及熟地黄饮片的供试品溶液制备方法和最佳薄层色谱条件。结果:采用薄层色谱高效硅胶G板,以正丁醇-甲醇-三氯甲烷-冰乙酸-水(13∶5∶5∶1∶2)为展开剂展开,喷以苯胺-二苯胺-磷酸溶液,110℃下加热显色,于日光底部灯下进行检视,所得地黄不同炮制品薄层色谱分离效果和显色效果较佳,斑点清晰且特征性好。结论:该薄层色谱鉴别方法操作简便易行,定性特征明显、结果直观,可有效鉴别地黄不同炮制品,并可为熟地黄的炮制终点确定提供实验依据。Objective: To establish a thin layer chromatography(TLC)identification method for different processed products of Rehmanniae Radix. Method: Catalpol, D-fructose, sucrose, raffinose,stachyose,melibiose and manninotriose were used as control substances,and the effects of extraction solvents(water,20% methanol,50% methanol,80% methanol),developing solvents(n-butanol-methanol-chloroformglacial acetic acid-water,ethyl acetate-pyridine-glacial acetic acid-water,n-butanol-glacial acetic acid-water),color reagents(aniline-diphenylamine-phosphoric acid solution,ninhydrin solution),sampling volumes(2,4,6 μL)and inspection conditions(sunlight, bottom lamp of sunlight, 365 nm, 254 nm)on TLC were investigated to determine the preparation method of sample solution of Rehmanniae Radix and Rehmanniae Radix Praeparata and the optimal TLC conditions. Result: High performance silica gel G plate was used for TLC,n-butanol-methanol-chloroform-glacial acetic acid-water(13∶5∶5∶1∶2)was used as developing agent,aniline-diphenylamine-phosphoric acid solution was sprayed and heated at 110 ℃ for color development,and then inspected under the bottom lamp of sunlight. The separation and color development of different processed products of Rehmanniae Radix were good with clear spots and good characteristics. Conclusion: The established TLC is simple and easy to operate with obvious qualitative characteristics and intuitive results. It can effectively identify different processed products of Rehmanniae Radix and provide experimental basis for determining the end point of processing of Rehmanniae Radix Praeparata.

关 键 词：地黄 熟地黄 薄层色谱法 展开剂 显色剂 糖类 提取溶剂 

分 类 号：R22[医药卫生—中医基础理论] O657[医药卫生—中医学]