沉默Rbfox1对癫痫模型大鼠海马神经细胞生物学行为的影响及机制研究  

The effect and mechanism of Rbfox 1 silencing on the biological behavior of hippocampal neurons in epileptic rats

作　　者：冯磊 解利平 张海平 束坤 Feng Lei;Xie Liping;Zhang Haiping;Shu Kun(Department of Neurosurgery, Xidian Group Hospital, Shaanxi Province,Xi’an 710000, China)

机构地区：[1]西电集团医院神经外科,西安710000

出　　处：《疑难病杂志》2020年第8期835-838,共4页Chinese Journal of Difficult and Complicated Cases

基　　金：陕西省西安市医疗卫生科研项目(201766984)。

摘　　要：目的研究沉默结合RNA的Fox1基因(Rbfox1)对癫痫模型大鼠海马神经细胞生物学行为的影响及其机制。方法2019年8月—10月于西电集团医院神经外科实验室进行实验,选择SD健康雄性大鼠60只,随机选取其中15只作为空白对照组,其余45只建立癫痫模型,按随机数字表法分为模型组、过表达组、沉默组各15只,构建Rbfox1过表达、沉默慢病毒载体,行慢病毒滴定,比较4组大鼠海马神经细胞增殖、凋亡情况及其Akt/mTOR通路蛋白表达量。结果大鼠不同时间点海马神经细胞凋亡率比较,过表达组>模型组>沉默组(F/P=17.500/0.001、7.217/0.001、5.243/0.001),增殖率比较,过表达组<模型组<沉默组(F/P=37.165/0.001、23.632/0.001、3.651/0.001),大鼠海马组织p-p38、p-JNK、p-ERK、Bax、Bim比较,过表达组>模型组>沉默组,Bcl-2比较,过表达组<模型组<沉默组(F/P=53.319/0.001、19.523/0.001、9.580/0.001、3.550/0.001、8.107/0.001、4.770/0.001)。结论沉默Rbfox1基因可抑制海马神经细胞凋亡,促进海马神经细胞增殖,其作用机制可能与调控MAPK通路相关。Objective To study the effect and mechanism of rffox 1 gene silencing on the biological behavior of hippocampal neurons in epileptic rats.Methods From August to October 2019,the experiment was carried out in Neurosurgery Laboratory of Xidian Group Hospital,and 60 SD healthy male rats were selected.Fifteen of them were randomly selected as the blank control group,and the other 45 were randomly divided into model group,over expression group and silence group.Construction of rbfox 1 over expression,silencing lentivirus vector,lentivirus titration.The proliferation and apoptosis of hippocampal neurons and the expression of Akt/mTOR pathway protein in the four groups were compared.Results Compared the apoptosis rate of hippocampal neurons at different time points in rats,overexpression group>model group>silence group(F/P=17.500/0.001,7.217/0.001,5.243/0.001),proliferation rate was compared,overexpression group<model group<silence group(F/P=37.165/0.001,23.632/0.001,3.651/0.001).Compared with p38,p-JNK,p-ERK,Bax and Bim in hippocampus of rats,overexpression group>model group>silence group;compared with Bcl-2,overexpression group<model group<silence group(F/P=53.319/0.001,19.523/0.001,9.580/0.001,3.550/0.001,8.107/0.001,4.770/0.001).Conclusion Rbfox1 gene silencing can inhibit the apoptosis of hippocampal neurons and promote the proliferation of hippocampal neurons.The mechanism may be related to the regulation of MAPK pathway.

关 键 词：结合RNA的Fox1基因 癫痫 海马神经细胞 细胞生物学行为 

分 类 号：R74[医药卫生—神经病学与精神病学]