腹外侧中脑导水管灰质多巴胺受体参与丙泊酚麻醉的作用研究  

Dopamine D1 receptor in ventrolateral periaqueductal gray modulates propofol anesthesia in rats

作　　者：张益 杨银银 熊以强 蒲青 赵燕飞 刘程曦 ZHANG Yi;YANG Yin-yin;XIONG Yi-qiang;PU Qing;ZHAO Yan-fei;LIU Cheng-xi(Department of Anesthesiology,the Second Affiliated Hospital of Zunyi Medical University,Zunyi 563000,Guizhou,China;Department of Anesthesiology,Guizhou Key Laboratory of Anesthesia and Organ Protection,Zunyi Medical University,Zunyi 563000,Guizhou,China)

机构地区：[1]遵义医科大学第二附属医院麻醉科,遵义563000 [2]遵义医科大学贵州省麻醉与器官保护基础研究重点实验室,遵义563000

出　　处：《医学研究生学报》2020年第8期792-796,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81560237);遵义医学院大学生创新创业训练计划项目(遵医201751004)。

摘　　要：目的腹外侧中脑导水管(vlPAG)是位于上行网状系统的重要调控睡眠觉醒区域,但其在麻醉中的作用尚未明确。文章拟借助光纤钙信号记录、微注射和在体脑电记录观察腹外侧中脑导水管灰质多巴胺受体对丙泊酚麻醉的影响。方法10只健康雄性SD大鼠于腹外侧中脑导水管灰质处微注射钙信号病毒并埋植记录光纤,观察丙泊酚麻醉诱导和苏醒过程中vlPAG神经元活性变化。建立50只SD大鼠中vlPAG双侧微注射模型,随机数字表法分D1R激动剂组、D1R拮抗剂组、D2R激动剂组、D2R拮抗剂组及对照组,每组10只。在丙泊酚麻醉下,5组分别于vlPAG分别双侧微注射1μL的D1R激动剂、D1R拮抗剂、D2R激动剂、D2R拮抗剂和等渗盐水,观察大鼠麻醉诱导和苏醒时间以及给药前后各个频段脑电图(δ波:1~4 Hz,θ波:4~8 Hz,α波:8~12 Hz,β波:12~25 Hz,γ波:25~60 Hz)的变化。结果与诱导前基线相比,诱导期、麻醉早期和麻醉期钙信号均显著降低(P<0.05)。与麻醉维持期基线相比,苏醒早期和苏醒期钙信号显著增加(P<0.05)。D1R激动剂组、D1R拮抗剂组丙泊酚麻醉的诱导时间[(886.7±124.5)、(506.7±90.7)s]较对照组[(659.3±103.5)s]延长(P<0.05),苏醒时间[(702.0±109.7)、(1068.0±69.0)s]较对照组[(901.3±130.4)]缩短(P<0.05)。与给药前相比,D1R激动剂组大鼠δ频段的能量明显降低(P<0.05);D1R抑制剂组δ频段的能量显著增加(P<0.05),同时β频段的能量明显增高(P<0.05)。结论中脑导水管腹外侧灰质通过多巴胺D1R受体参与调控丙泊酚麻醉过程。Objective Ventrolateral periaqueductal gray(vlPAG)locates in ascending reticular activating system,which plays a key role in the sleep-wake circle.However,the role of vlPAG in general anesthesia has not been identified.To investigate the effect of the dopamine receptor in vlPAG neurons on propofol anesthesia,we used real-time in vivo fiber photometry,microinjection and EEG.Methods To observe the alteration of neuronal activity in the vlPAG throughout propofol anesthesia,10 Sprague-Dawley rats were used for calcium fiber photometry recording.50 vlPAG bilateral microinjection models were established and assigned into five groups randomly,including D1R agonist group,D1R antagonist group,D2R agonist group,D2R antagonist group,and control group(n=10).Under propofol anesthesia,1μL of D1R agonist,D1R antagonist,D2R agonist,D2R antagonist,and isotonic saline were microinjected into the vlPAG of animals in the corresponding groups,respectively.The induction time,recovery time and the changes in electroencephalogram(EEG)before and after microinjection were recorded and analyzed.Results The neuronal activity in the vlPAG was significantly inhibited during the induction period and markedly recovered during the recovery period from propofol anesthesia(P<0.05).Subsequently,the microinjection of D1R agonist into the vlPAG notably prolonged the induction time and reduced the emergence time of propofol anesthesia with a decrease ofδ-band ratio.While the microinjection of D1R antagonist accelerated the induction time and prolonged the emergence time of propofol anesthesia with an increase ofδ-band ratio and a decrease inβ-band ratio in cortical EEG(P<0.05).The induction and recovery time of D2R agonist/antagonist group did not differ with those of control group.As well,EEG before and after microinjection in D2R agonist/antagonist group did not different.Conclusion These results indicate that vlPAG modulates the process of propofol anesthesia via D1R.

关 键 词：腹外侧中脑导水管灰质 丙泊酚麻醉 D1受体 D2受体 光纤钙信号记录 

分 类 号：R74[医药卫生—神经病学与精神病学]