单细胞质谱分析方法研究进展  

Recent Advances in Single Cell Analysis Methods Based on Mass Spectrometry

作　　者：王举铎 宋佳峰 李畅 戴新华[2] 方向[2] 龚晓云[2] 叶子弘 WANG Ju-Duo;SONG Jia-Feng;LI Chang;DAI Xin-Hua;FANG Xiang;GONG Xiao-Yun;YE Zi-Hong(College of Life Sciences,China Jiliang University,Hangzhou 310018,China;Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029,China)

机构地区：[1]中国计量大学生命科学学院,杭州310018 [2]中国计量科学研究院前沿计量科学中心,北京100029

出　　处：《分析化学》2020年第8期969-980,共12页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21605135,21927812)资助。

摘　　要：细胞的个体差异性对于生物体生理功能的运行至关重要,准确阐释相关生物学机理需要以单细胞化学组分的准确测量为基础。然而,由于单细胞中的物质含量低、组成复杂,且不同物质的含量差异大,导致单细胞分析的技术难度极大。质谱技术凭借其高灵敏度、高特异性,以及强大的结构解析能力,近年来在单细胞分析研究中得到了广泛应用。研究者已建立了多种质谱分析方法,实现了单细胞中不同物质的准确测量。从离子化技术的角度,单细胞质谱分析方法目前主要包括三类,即纳升电喷雾离子化质谱法、激光解吸附离子化质谱法和二次离子质谱法。本文对近年基于上述三种离子化技术的单细胞质谱分析方法的研究进展进行了归纳和总结,对单细胞质谱分析方法未来的发展趋势进行了展望。The heterogeneity among individual cells is of great importance for organisms to achieve different biological functions.Investigations deep into the mechanism of some biological phenomena rely on the analysis of the chemical compositions of single cells.Unfortunately,the successful analysis of single cells is technically difficult to achieve due to the complex composition of single cells,the very limited amount of materials in single cells and the huge difference among different metabolites in their concentrations.Mass spectrometry(MS)has been widely used in single cell analysis in recent years due to its high sensitivity,high specificity and capability for structure identification.Methods based on MS have been developed for the analysis of different metabolites in single cells.From the perspective of ionization techniques,there are mainly three single cell analysis methods,including nano-electrospray ionization(Nano-ESI)based method,laser desorption ionization(LDI)based method and secondary ion MS(SIMS)based method.This review summarizes the up-to-date developments of these methods and their future trends.

关 键 词：质谱 单细胞分析 离子化 纳升电喷雾离子化 激光解吸附离子化 二次离子质谱 评述 

分 类 号：O657.63[理学—分析化学] Q2-33[理学—化学]