金纳米棒免疫层析试纸条定量检测前列腺特异性抗原  

Gold Nanorods-based Immunochromatographic Strip for Rapid and Quantitative Detection of Prostate Specific Antigen

作　　者：张婳[2] 李群[1] 刘桂锋[1] ZHANG Hua;LI Qun;LIU Gui-Feng(Department of Radiology,China-Japan Union Hospital of Jilin University,Changchun 130033,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)

机构地区：[1]吉林大学中日联谊医院放射线科,长春130033 [2]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022

出　　处：《分析化学》2020年第8期1018-1024,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.61901438)资助。

摘　　要：建立了一种基于金纳米棒(GNR)的免疫层析方法,用于快速定量检测前列腺特异性抗原(PSA)。以GNR为标记探针,通过包裹羧基化的聚合物后,采用共价偶联的方式连接抗PSA单克隆抗体形成偶联物,以鼠抗PSA单克隆抗体和羊抗鼠IgG分别喷涂硝酸纤维素膜,形成试纸条的检测线和质控线,采用三明治夹心法构建GNR免疫层析试纸条,用于PSA的定量检测。结果表明,GNR免疫层析试纸条特异性强,稳定性好,灵敏度高,对PSA检测的线性范围为0.1~50 ng/mL,检出限为0.1 ng/mL,批内和批间变异系数均小于10%,且具有良好的保存稳定性。本方法可用于检测血清中PSA的浓度水平,对于前列腺癌的早期诊断、监测治疗及预后判断具有重要意义。A gold nanorod(GNR)-based immunochromatographic strip was developed for rapid and quantitative detection of prostate specific antigen(PSA).GNR was used as the labeled probe and coated with sodium polyacrylate.Then the anti-PSA monoclonal antibody(dAb)was immobilized on the surface of GNR through covalent attachment to form the GNR-dAb conjugates.The mouse anti-PSA monoclonal antibody and the goat anti-mouse IgG were sprayed onto the nitrocellulose membrane as test line and control line,respectively.The resultant GNR-dAb conjugates were introduced to construct the immunochromatographic strip for the quantitative detection of PSA by a sandwich method.The proposed GNR-based immunochromatographic strip exhibited good specificity,high stability and high sensitivity.The experimental results indicated that GNR-based immunochromatographic strip showed a good linear range from 0.1 ng/mL to 50 ng/mL with a limit of detection of 0.1 ng/mL,and the relative standard deviation was less than 10%.The established assay could be successfully applied for PSA detection in serum,which was beneficial for early diagnosis,treatment and prognosis of prostate cancer.

关 键 词：金纳米棒 免疫层析 前列腺特异性抗原 定量检测 

分 类 号：R737.25[医药卫生—肿瘤] R446.6[医药卫生—临床医学]