水曲柳FmMYB转录因子生物信息学及表达模式分析  

Bioinformatic and Expression Pattern Analyses of FmMYB Transcription Factor in Fraxinus mandshurica Rupr.

作　　者：马铭浩 张晗 肖英 刘华领 周姗[1] 詹亚光[1] 曾凡锁[1] MA Ming-hao;ZHANG Han;XIAO Ying;LIU Hua-ling;ZHOU Shan;ZHAN Ya-guang;ZENG Fan-suo(College of Life Sciences,Northeast Forestry University,Harbin 150040,China;Heilongjiang Shanhetun Forestry Bureau,Harbin 150001,China)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]黑龙江省山河屯林业局,黑龙江哈尔滨150001

出　　处：《黑龙江农业科学》2020年第7期21-28,共8页Heilongjiang Agricultural Sciences

基　　金：黑龙江省应用技术研究与开发计划项目(GA19B201);东北林业大学大学生创新项目(201810225337)。

摘　　要：MYB蛋白广泛存在于植物中,参与植物生长发育等多种代谢途径。前期研究获得2个FmMYB转录因子cDNA全长序列(FmMYB2和FmMYB20),已上传至NCBI并获得登录号(KT261341和KT261342)。为揭示FmMYB转录因子功能,对这两个MYB转录因子的结构特征、生化特性进行分析,利用实时荧光定量PCR,对FmMYB2和FmMYB20的时空表达模式以及施加应力后的转录水平进行分析。结果表明:FmMYB转录因子的时空表达模式有所差异,FmMYB2基因在7月相对表达量最高,约为最低值5月的60倍;然而在8月,FmMYB20基因的相对表达量最高,约为最低值5月的5倍;同时在叶、木质部、皮3个部位中,FmMYB2和FmMYB20基因在木质部中表达量最高。FmMYB2与FmMYB20基因均对应拉处理有响应,FmMYB20与木质素合成相关基因4CL表达模式相似。研究结果初步说明FmMYB2和FmMYB20可能参与木质素的合成。MYB protein is widely present in plants and participates in various aspects of plant primary and secondary metabolic reactions.The structure characteristics of MYB transcription factors in Fraxinus mandshurica Rupr.were studied.Two full-length FmMYB transcription factor(FmMYB2 and FmMYB20)were obtained,which were uploaded to the NCBI and got the accession number(KT261341 and KT261342).The spatiotemporal expression patterns and the mechanism of response to gravity were revealed.Using bioinformatics software,FmMYB and its encoding protein were analyzed in detail.FmMYB amino acid sequence phylogenetic tree was constructed.The expression pattern of FmMYB transcription factor was detected by real-time fluorescence quantitative PCR.The results showed that FmMYB2 was highest in July,about 50 times of May′s,while FmMYB20 gene was highest in August,about 5 times of May′s;The expressions of FmMYB were highest in xylem.Expression patterns of FmMYB transcription factor were different.These results showed the expressions of FmMYB transcription factor were spatiotemporal specificity.There were differences between the 2 genes in response to gravity treatment,which suggested FmMYB transcription factor might be involved in lignin synthesis.

关 键 词：水曲柳 MYB 时空表达 应拉木 生物信息学分析 

分 类 号：R73[医药卫生—肿瘤]