川西高原野生黑木耳胶质的提取及成分测定  

Extraction and Determination of Components of Wild Auricularia auricula colloid from Western Sichuan Plateau

作　　者：杜亚飞 邹翠 曹玉 刘绪 徐坤[1,2] DU Yafei;ZOU Cui;CAO Yu;LIU Xu;XU Kun(College of Chemistry and Life Sciences,Chengdu Normal University;Food Fermentation Institute,Chengdu Normal University,ChengDu SiChuan,611130,China)

机构地区：[1]成都师范学院化学与生命科学学院 [2]成都师范学院食品发酵研究所,四川成都611130

出　　处：《高原农业》2020年第4期417-421,428,共6页Journal of Plateau Agriculture

基　　金：成都师范学院校级青年项目(2019CS19ZC05),野生黑木耳胶质的提取及成分测定。

摘　　要：本研究以川西高原地区的野生黑木耳作为研究对象,采用热水浸提法除去多糖,保留黑木耳胶质,以胶质的提取率为指标,通过单因素和正交试验,考察了热水浸提温度、料液比以及浸提时间对于胶质提取率的影响,并对工艺条件进行了优化,最终确定最优的工艺条件为:料液比1∶45 g/mL,浸提时间3.0 h,浸提温度75℃,在此条件下黑木耳胶质的提取率最低,为70.28%。对该条件下得到的胶质进行了成分测定,其中总糖含量为36.14%,蛋白质含量为9.82%,灰分为4.28%,粗纤维含量为2.76%,脂肪含量为0.47%。In this study,wild Auricularia auricula from Western Sichuan Plateau was studied,and hot water leaching method was used to remove the polysaccharides to keep the colloid.Taking the extraction ratio of colloid as an index,the effects of extraction temperature,material-liquid ratio and extraction time on extraction ratio of colloid were investigated through single factor test and orthogonal test,while the process conditions were optimized,and the optimal process conditions were determined as follows:material-liquid ratio 1:45(g/mL),leaching time 3.0h,leaching temperature 75℃.Under this condition,the extraction ratio of colloid was 70.28%.The main components of the colloid were determined,showing that the total sugar content was 36.14%;the protein content was 9.82%;the ash content was 4.28%;the crude fiber content was 2.76%and the fat content was 0.47%.

关 键 词：野生黑木耳 木耳胶质 热水浸提法 成分测定 

分 类 号：TS201.3[轻工技术与工程—食品科学]