低氧诱导因子-1α通过提高miR-210-3p表达水平促进口腔鳞状细胞癌细胞的增殖和迁移  

HIF-1α Promotes Migration and Proliferation of Oral Squamous Cell Carcinoma Cell by Increasing the Expression Level of MiR-210-3p

作　　者：王慧[1] 尚政军[1] WANG Hui;SHANG Zhengjun(The State Key Laboratory Breeding Base of Basic Science of Stomatology,Hubei Province&Key Laboratory of Oral Biomedicine,Wuhan University,Ministry of Education,Wuhan 430072,Hubei Province,China)

机构地区：[1]湖北省口腔基础医学重点实验室·省部共建国家重点实验室培育基地,武汉大学口腔生物医学教育部重点实验室,湖北武汉430072

出　　处：《口腔颌面外科杂志》2020年第4期201-205,共5页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金面上项目(81972547);湖北省自然科学基金(2019CFB503)。

摘　　要：目的:研究低氧诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)是否通过改变miR-210-3p的表达水平影响口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)的进展。方法:低氧培养CAL-27细胞并检测细胞内HIF-1α、miR-210-3p的表达水平;重组HIF-1α刺激CAL-27细胞后检测miR-210-3p的表达水平是否发生变化;转染短发夹RNA(short hairpin RNA,shRNA)改变CAL-27细胞内miR-210-3p的表达水平后,用划痕实验和CCK-8实验来观察其对CAL-27细胞迁移、增殖能力的影响。结果:低氧导致CAL-27细胞内HIF-1α及miR-210-3p表达量增高;重组HIF-1α处理后的CAL-27细胞内miR-210-3p表达量升高;过表达miR-210-3p会促进CAL-27细胞的增殖及迁移能力,反之细胞的增殖和迁移能力则受到抑制。结论:MiR-210-3p在OSCC细胞增殖和迁移过程中起到重要作用,HIF-1α通过影响miR-210-3p表达来参与肿瘤进程。Objective:To study whether hypoxia-inducible factor 1α(HIF-1α)affects the progress of oral squamous cell carcinoma(OSCC)by changing the expression level of miR-210-3p.Methods:OSCC CAL-27 cell line was cultured with hypoxia and the expression level of HIF-1αand miR-210-3p was detected.After stimulated by recombinant HIF-1α,we detected the expression level of miR-210-3p in CAL-27 cells.And the effects of short hairpin RNA(shRNA)on the migration and proliferation of CAL-27 cells were observed by Scratch and CCK-8 assay after shRNA transfer changed the expression level of miR-210-3p in CAL-27 cells.Results:Hypoxia condition increased the expression level of HIF-1αand miR-210-3p in CAL-27 cells.The expression intensity of miR-210-3p increased in CAL-27 cells treated with recombinant HIF-1α.Overexpression of miR-210-3p promoted the proliferation and migration of CAL-27 cells,while the proliferation and migration of CAL-27 cells were inhibited by reproduction.Conclusion:MiR-210-3p plays an important role in the proliferation and migration of OSCC cells,and HIF-1αparticipates in the tumor process by affecting the expression level of miR-210-3p.

关 键 词：口腔鳞状细胞癌 miR-210-3p 低氧诱导因子-1Α 迁移 

分 类 号：R782[医药卫生—口腔医学] R739.8[医药卫生—临床医学]