脑片培养技术及其在药物研发中的应用进展  

Progress of Brain Slice Culture Technology and Its Application in Drug Research and Development

作　　者：李伟瀚 王月华[1] 杜冠华[1] LI Wei-han;WANG Yue-hua;DU Guan-hua(Beijing Key Laboratory of Drug Target Identification and New Drug Screening,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China)

机构地区：[1]药物靶点研究与新药筛选北京市重点实验室,中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《中国药学杂志》2020年第13期1068-1071,共4页Chinese Pharmaceutical Journal

基　　金：国家重点研发计划项目资助(2018YFC0311005);国家自然科学基金面上项目资助(81473383);国家科技重大专项重大新药创制项目资助(2018ZX09711001-003-019);中国医学科学院医学与健康科技创新工程项目资助(2016-I2M-3-007)。

摘　　要：脑片培养是一种离体研究脑内神经细胞分子机制及电生理活动的重要实验技术,是研究脑内生理病理过程的重要工具。近年来,随着脑片培养技术及检测方法的不断提高,脑片存活时间大大延长,检测手段更加多样化,脑片培养技术在生物医药领域应用范围不断扩大。笔者综述脑片培养技术的进展,讨论脑片培养技术在神经疾病模型建立及药物研发中的应用,脑片培养技术对神经科学研究及药物研发具有重要意义。Brain slice culture is an important experimental technique for studying the molecular mechanism and electrophysiological activities of brain ex vivo.It is also an important tool for studying the physiological and pathological processes in brain.Recently,with the continuous improvement of brain slice culture technology and detection methods,the survival time of brain slices has been greatly prolonged,monitoring methods have become more diversified,and the application of brain slice culture technology has been expanding in biomedical field.In this article,the progress of brain slice culture technology is reviewed.The application of brain slice culture technology in the establishment of neurological disease model and drug development is discussed.Brain slice culture technology is of great significance to neuroscience research and drug development.

关 键 词：脑片培养 药物发现 神经科学 神经退行性疾病 

分 类 号：R965[医药卫生—药理学]