高选择性检测Hg2+的喹啉酮衍生物荧光探针的合成及应用  

Synthesis and Application of Quinolinone Derivative Fluorescent Probe for High Selective Detection of Hg

作　　者：张成路[1] 孙越冬 王静 何钰 张彦鹏 张璐 宋府璐 ZHANG Chenglu;SUN Yuedong;WANG Jing;HE Yu;ZHANG Yanpeng;ZHANG Lu;SONG Fulu(College of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian 116029,China;Sinosteel Anshan Research Institute of Thermo-energy Co.,Ltd,National Local United Engineering Research Center for Preparing Technology of Coal-tar Based New Materials,Anshan 114044,China)

机构地区：[1]辽宁师范大学化学化工学院,大连116029 [2]中钢集团鞍山热能研究院有限公司,煤焦油系新型材料制备技术国家地方联合工程中心,鞍山114044

出　　处：《高等学校化学学报》2020年第8期1785-1791,共7页Chemical Journal of Chinese Universities

基　　金：辽宁省教育厅科学技术资助项目(批准号:2009A426)资助。

摘　　要：设计合成了一种以喹啉酮为荧光团,具有新型结构的荧光探针(E)-N-(4-甲基-2-氧代-1,2-二氢喹啉-7-基)-3-(3-苯基-[1,2,4]三唑[3,4-b][1,3,4]噻二唑-6-基)丙烯酰胺(MNT).研究结果表明, MNT可通过不饱和酰胺键异构化后与Hg2+配位,从而产生显著的荧光猝灭.1,2,4-三唑[3,4-b]-1,3,4-噻二唑缺电子的特征有助于提高猝灭效果的同时,能提供与Hg2+配位的杂原子.MNT探针对Hg2+具有高选择性、较高的量子产率和较强的抗干扰性,检测限为6.35×10-8mol/L,响应时间25 s.进一步研究发现, MNT在pH=4~13范围内均能特异性检测Hg2+.基于核磁滴定实验结果推测了该探针荧光检测Hg2+的机理,并由Job’s曲线确定了MNT与Hg2+之间的配位比为2∶1.MNT在实际水样中的应用结果表明其可作为检测Hg2+的荧光探针.In order to well-directed fluorescence recognize and detect Hg2+,according to the coordination characteristics of Hg2+,a novel fluorescent probe(E)-N-(4-methyl-2-oxo-1,2-dihydroquinolin-7-yl)-3-(3-phenyl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol-6-yl)acrylamide(MNT)was designed and synthesized,which quinolinone block was used as fluorophore.The results showed that MNT could coordinate with Hg2+after the isomerization of amide bond,therefore the fluorescence quenching was generated.1,2,4-Triazole[3,4-b]-1,3,4-thiadiazole had the characteristic of lacking electrons,which could improve the quenching effect and coordinate with Hg2+.The probe MNT had higher selectivity,higher quantum yield and stronger antiinterference for Hg2+.The detection limit was 6.35×10-8 mol/L,the response time was 25s.The research also found that MNT could determine Hg2+in the range of pH 4—13.The fluorescence detection mechanism was speculated through nuclear magnetic titration experiments and the coordination ratio 2:1 was determined by Job’s curve.The results of the application in practical water samples indicated that MNT could be used as a fluorescent probe for Hg2+.

关 键 词：喹啉酮 1 2 4-三唑[3 4-b]-1 3 4-噻二唑 荧光识别 Hg2+ 

分 类 号：O626[理学—有机化学] O657.3[理学—化学]