咀嚼对应激大鼠杏仁核和前额叶皮质中GAT-1的影响  

Effects of Chewing on GAT-1 in Amygdala and Frontal Cortex of Stressed Rats

作　　者：杨佳迪 边策 王旭[1] 李荷香[1] YANG Jiadi;BIAN Ce;WANG Xu;LI Hexiang(Department of Basic Teaching and Research, School of Hospital of Stomatology, Hebei Medical University & Hebei Key laboratory of Stomatology, Shijiazhuang 050017, China;Department of Orthodontics, Beijing Hospital of Stomatology, Capital Medical University, Beijing 100050, China)

机构地区：[1]河北医科大学口腔医学院·口腔医院基础教研室,河北省口腔医学重点实验室,河北石家庄050017 [2]首都医科大学附属北京口腔医院正畸科,北京100050

出　　处：《口腔医学研究》2020年第8期736-740,共5页Journal of Oral Science Research

摘　　要：目的:探讨咀嚼对应激大鼠杏仁核、前额叶皮质GABA再摄取的影响。方法:将成年雄性SD大鼠52只分成对照组(CO组)、应激组(ST组)、应激+咀嚼组(SC组)。除CO组外,各组大鼠均置于固定筒中束缚30 min。造模同时,SC组给予直径5 mm木棍以供其咀嚼,咀嚼少于15 min者被丢弃。通过旷场实验记录各组大鼠运动得分以测试其焦虑程度,实验结束后随机挑选大鼠18只(每组6只)立即处死,取杏仁核、前额叶皮质组织检测γ-氨基丁酸转运体-1(GAT-1)mRNA表达情况。结果:与CO组相比,ST组大鼠OF值显著降低,杏仁核与前额叶皮质GAT-1 mRNA表达显著升高(P<0.05);SC组大鼠OF值较ST组显著升高,杏仁核与前额叶皮质GAT-1 mRNA表达显著降低(P<0.05),但与CO组比较差异无统计学意义(P>0.05)。结论:咀嚼可以通过纠正杏仁核、前额叶皮质GAT-1的异常表达调节GABA代谢失常而发挥缓解压力的作用。Objective:To investigate the effect of mastication on GABA reuptake in amygdala and frontal cortex of rats.Methods:52 SD rats were divided into control group(CO group),stress group(ST group),and stress+chewing group(SC group).Except for CO group,rats in each group were placed in a fixed tube for 30 minutes.Meanwhile,the SC group was given a 5mm diameter stick for chewing,and those chewing for less than 15 minutes were discarded.The motor scores of each group were recorded in Open-Field test.At the end of the experiment,18 rats(6 in each group)were randomly selected and killed immediately.Results:Compared with CO group,the OF value of ST group was significantly lower,and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly higher(P<0.05).The OF value of SC group was significantly higher than ST group,and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly lower(P<0.05).There was no significant difference between SC group and CO group(P>0.05).Conclusion:Mastication can relieve the stress by correcting the abnormal expression of GAT-1 in amygdala and frontal cortex.

关 键 词：咀嚼 γ-氨基丁酸 Γ-氨基丁酸转运体 应激衰减效应 

分 类 号：R74[医药卫生—神经病学与精神病学]