下颌骨髁突高位骨折游离复位愈合过程中DKK-1/BMP-2的表达研究  

Expression of DKK-1/BMP-2 in the Healing of Mandibular High Condylar Fracture

作　　者：王冬香 王光素 唐正龙 陈友利 高琼 安宁 WANG Dongxiang;WANG Guangsu;TANG Zhenglong;CHEN Youli;GAO Qiong;AN Ning(Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Guizhou Medical University, Guiyang 550004, China)

机构地区：[1]贵州医科大学附属口腔医院口腔颌面外科,贵州贵阳550004

出　　处：《口腔医学研究》2020年第8期748-752,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(81460101);贵州省科学技术基金(黔科合基础[2017]1151)。

摘　　要：目的:探索下颌骨髁突高位骨折游离复位愈合过程中Dickkopf-1(DKK-1)和骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)的表达。方法:24只兔建立下颌骨髁突高位骨折实验动物模型,随机分为对照组和实验组。实验组剥离髁突骨膜软组织附着,对照组保留软组织附着,术后1、2、3、4周处死动物,HE染色观察新骨组织学形态变化;免疫组织化学法检测新骨组织中BMP-2和DKK-1的表达强度。结果:随固定时间的延长,对照组骨折区的骨愈合速度及质量优于实验组;骨折愈合过程中对照组BMP-2的表达均高于实验组,对照组DKK-1的表达低于实验组。结论:下颌骨髁突高位骨折游离复位固定术后骨愈合速度较慢,可能与高位骨折后血供不足和DKK-1表达升高有关。Objective:To explore the expression of Dickkopf-1(DKK-1)and bone morphogenetic protein-2(BMP-2)in the healing of mandibular high condylar fracture.Methods:The experimental animal model of high condylar fracture of mandible was established in 24 rabbits.The rabbits were randomly divided into control group and experimental group.The condylar periosteum soft tissue was removed in the experimental group but remained in the control group.The animals were killed separately in 1,2,3,and 4 weeks after operation,and the histomorphological changes of new bone was observed by HE staining,and the expression of BMP-2 and DKK-1 in new bone tissue was detected by immunohistochemistry.Results:With the extension of fixation time,the healing speed and quality of the fracture area in the control group were better than that in the experimental group.The expression of BMP-2 in the control group was higher than that in the experimental group,and the expression of DKK-1 in the control group was lower than that in the experimental group.Conclusion:The delayed bone healing after free reduction and fixation of high condylar fracture of mandible may relate to insufficient blood supply and increased expression of DKK-1.

关 键 词：下颌骨髁突 骨折愈合 骨形态发生蛋白 DICKKOPF-1 

分 类 号：R73[医药卫生—肿瘤]