不同pH条件下五倍子中β-PGG水解产物分析及其含量研究  

Analysis and Content Study ofβ-PGG Hydrolysate in Galla Chinensis under Different pH Conditions

作　　者：张艳娇 杨玲 向润清 黄宽 方山丹 范源 朱培芳 ZHANG Yan-jiao;YANG Ling;XIANG Run-qing;HUANG Kuan;FANG Shan-dan;FAN Yuan;ZHU Pei-fang(Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;The Second Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming 650216,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]云南中医药大学第二附属医院,云南昆明650216

出　　处：《云南中医中药杂志》2020年第8期54-58,共5页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：国家自然科学基金地区基金项目(81360652)。

摘　　要：目的研究并分析β-PGG、3GG、GA、PA等在不同pH磷酸盐缓冲液中的含量变化及β-PGG水解产物分析情况。方法采用经典恒温法考察β-PGG、GA、PA在不同pH缓冲液(pH=2、3、4、5、6、7、8)中的水解情况,用RP-HPLC法在同一色谱条件下对β-PGG水解产物进行分析及含量测定。结果在同一色谱条件下发现β-PGG分解为GA和少量3GG之外,同时考察了GA在不同缓冲溶液中的产物分析,发现GA可生成少量3GG及PGG,未见PA分解。结论β-PGG及GA、PA在不同pH条件下的含量变化反映了不同酸碱度对β-PGG水解反应的影响;本实验也发现β-PGG可分解为GA,3GG;GA在一定的条件下会合成3GG及β-PGG。目前文献多报道β-PGG的合成,其在不同PH条件下分解的产物及产物含量变化的研究报道较少,本研究有助于了解β-PGG的化学稳定性及其分解产物的相关性,可为进一步研究β-PGG的理化特性及鞣质类中药的养护、储存、加工及临床应用提供一定参考。Objective:To study and analyze the content changes andβ-PGG hydrolysates ofβ-PGG,3GG,GA and PA in different pH phosphate buffers.Methods:The classic constant temperature method was used to investigate the hydrolysis ofβ-PGG,GA,and PA in different pH buffers(pH=2,3,4,5,6,7,and 8).The RP-HPLC method was used under the same chromatographic conditions.Results:Under the same chromatographic conditions,it was found thatβ-PGG was decomposed into GA and a small amount of 3GG.At the same time,the product analysis of GA in different buffer solutions was investigated and GA was found to generate a small amount of 3GG and PGG,and no PA decomposition was seen.Conclusion:The content changes ofβ-PGG,GA and PA under different pH conditions reflect the influence of different pH on the hydrolysis reaction ofβ-PGG.This experiment also find thatβ-PGG can be decomposed into GA and 3GG and GA under certain conditions can synthesize 3GG andβ-PGG.At present,the literature mostly reports the synthesis ofβ-PGG,and few research reports on its decomposition products and product content changes under different pH conditions.This study helps to understand the chemical stability ofβ-PGG and the correlation of its decomposition products and can provide a reference for further research on the physical and chemical properties ofβ-PGG and the maintenance,storage,processing and clinical application of tannin Chinese medicine.

关 键 词：β-PGG 1 3 6-Tri-O-galloylglucose 没食子酸 焦性没食子酸 pH缓冲液 水解反应 

分 类 号：R285[医药卫生—中药学]