‘中黄1号’茶树带腋芽茎段组培快繁体系建立  

Establishment of Tissue Culture System of Stem Segment of Tea Plant of’Zhonghuang No.1’

作　　者：谢恩俊 田维丽 陈正武 李岩 赵德刚 Xie Enjun;Tian Weili;Chen Zhengwu;Li Yan;Zhao Degang(The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Institute of Agro-Bioengineering,College o f Life Sciences,Guizhou University,Guiyang,550025;Tea Research Institute of Guizhou Academy of Agricultural Sciences,Guiyang station for DUS Testing Center of New Plant Varieties of MOA.PR.China in Guizhou Academy of Agricultural Sciences,Guiyang,550006)

机构地区：[1]贵州大学生命科学学院农业生物工程研究院山地植物资源保护与种质创新省部共建教育部重点实验室,贵阳550025 [2]贵州省农业科学院茶叶研究所贵州省农业科学院国家农业农村部DUS中心贵阳分中心,贵阳550006

出　　处：《分子植物育种》2020年第15期5071-5080,共10页Molecular Plant Breeding

基　　金：贵州省高层次创新人才培养项目(黔科合人才(2016)4003号);贵州鸟王茶古茶树组培快繁体系建立及人工种子技术研究(黔科合LH字(2017)7272号);贵州省科技基金项目(黔科合基础(2018)1044号);贵州省农业厅动植物育种专项项目(黔农育种专项字(2013)009号)共同资助。

摘　　要：本研究以‘中黄1号’茶树带腋芽茎段为外植体,探索外植体的取样时间和消毒条件、最佳萌发和增殖培养基、生根和壮苗培养基配方以及炼苗移栽条件。结果表明:最佳外植体取样时间为4月份,茎段经75%酒精浸泡60 s,0.1%升汞浸泡12 min,外植体存活率为91.3%,污染率为2.7%,以MS+2 mg/L 6-BA+1 mg/L GA3作为腋芽萌发培养基,培养40 d出芽率为91.7%,以MS+4 mg/L 6-BA+1 mg/L IBA作为增殖培养基,培养45 d增值系数可达3.8。以MS+1 mg/L 6-BA+0.5 mg/L IBA作为壮苗培养基进行壮苗,经45 d可培养至约4~5 cm后诱导生根,在50 mg/L IBA无菌溶液中浸泡5 min后,转至1/2MS+1.5 mg/L IBA生根培养基中,生根率可达82.5%。自然光条件下开瓶室温炼苗5 d,移栽到以营养土和蛭石2∶1比例基质中,成活率最高,移栽成活率为66.7%。本研究建立‘中黄1号’带腋芽茎段组培快繁体系,为‘中黄1号’茶树工厂化育苗提供技术支持。In this study,the stem segments of the tea buds of ’Zhonghuang No.1’ were taken as explants,and the sampling time and disinfection conditions of the explants,the best germination and proliferation medium,the rooting and strong seedling medium formula,and the seedling transplanting condition were explored.The results showed that the best explant sampling time was April,the stem segment was soaked in 75% alcohol for 60 s,0.1%mercury was immersed for 12 min,the explant survival rate was 91.3%,and the contamination rate was 2.7%.MS +2 mg/L 6-BA +1 mg/L GA3 was used as the axillary bud germination medium.The germination rate was91.7% after 40 days of culture.MS+4 mg/L 6-BA+1 mg/L IBA was used as the proliferation medium and cultured for 45 days.The value-added factor can reach 3.8.MS +1 mg/L 6-BA +0.5 mg/L IBA was used as the strong seedling medium for strong seedlings.After 45 days,it could be cultured to about 4~5 cm,and rooting was induced.Soaked in 50 mg/L IBA sterile solution.After 5 min,the cells were transferred to 1/2 MS+1.5 mg/L IBA rooting medium,and the rooting rate was 82.5%.Under natural light conditions,the bottle was incubated for 5 days at room temperature,and transplanted to a matrix with a ratio of nutrient soil and vermiculite in a ratio of 2:1.The survival rate was the highest,and the survival rate of transplanting was 66.7%.In this study,we established a tissue culture and rapid propagation system for the axillary bud stems of ’Zhonghuang No.1’,and provided technical support for the industrialized seedling cultivation of ’Zhonghuang No.1’ tea tree.

关 键 词：中黄1号 组培快繁 带腋芽茎段 

分 类 号：S571.1[农业科学—茶叶生产加工]