肥厚性幽门狭窄大鼠幽门中神经生长因子及其受体TrkA、p75NTR的表达  

Expression of nerve growth factor and its receptors TrkA and p75NTR in mice with hypertrophic pyloric stricture

作　　者：陈珊 陈文旭 徐迪[2] 李立帜[2] CHEN Shan;CHEN Wenxu;XU Di;LI Lizhi(Department of Clinical Laboratory,Fuzhou Second Hospital Affiliated to Xiamen University,Fuzhou,Fujian 350013,China)

机构地区：[1]厦门大学附属福州第二医院检验科,福州350013 [2]福建医科大学省立临床医学院福建省立医院小儿外科

出　　处：《福建医药杂志》2020年第4期134-136,共3页Fujian Medical Journal

摘　　要：目的研究肥厚性幽门狭窄大鼠幽门中神经生长因子(NGF)及神经营养因子酪氨酸激酶受体A(TrkA)、p75神经营养因子受体(p75NTR)表达情况,探讨NGF及其受体TrkA与p75NTR在先天性肥厚性幽门狭窄起病中的作用。方法将受孕后的SD大鼠随机分为模型组与对照组,模型组经尾静脉注射给予N-硝基-左旋精氨酸甲酯(L-NAME)20 mg/(kg·d),对照组经尾静脉注射给予同等剂量的生理盐水。新生鼠于出生后第1、7、14天与第21天分别称重,生后第2天麻醉处死,留取幽门组织。HE染色后显微镜下测量幽门肌层厚度。实时定量聚合酶链反应(RT-PCR)检测幽门组织中的NGF及其受体TrkA与p75NTR的mRNA表达水平。Western blot检测幽门组织中的NGF及其受体TrkA与p75NT的蛋白质表达水平。结果与对照组新生鼠比较,模型组新生鼠体质量增长明显缓慢,体质量较低。模型组幽门肌明显肥厚。模型组新生鼠NGF的mRNA及蛋白表达显著减少;模型组新生鼠TrkA与p75NTR的mRNA及蛋白表达显著减低,差异有统计学意义(P<0.05)。结论幽门组织中TrkA与p75NTR降低及NGF的表达减少可能与先天性肥厚性幽门狭窄的发病有关。Objective To study the expression of nerve growth factor(NGF), neurotrophic factor tyrosine kinase receptor A(TrkA) and p75 neurotrophic factor receptor(p75 NTR) in the pylorus of mice with hypertrophic pyloric stenosis, and to explore the role of NGF and its receptors TrkA and p75 NTR in the onset of congenital hypertrophic portal stenosis. Methods Pregnant SD mice were randomly divided into model group and control group. The model group was given 20 mg/(kg·d) of N-nitro-L-arginine methyl ester(L-NAME) by tail vein injection, while the control group was given the same dose of normal saline through tail vein. The newborn mice were weighed on the 1 st, 7 th, 14 th and 21 st day after birth. The mice were killed on the 2 nd day after birth. The thickness of pylorus muscle layer was measured under microscope after staining. Real time quantitative polymerase chain reaction(RT-PCR) was used to detect the mRNA expression levels of NGF, TrkA and p75 NTR in pyloric tissue. Western blot was used to detect the protein expression of NGF, TrkA and p75 NTR. Results Compared with the control group, the body weight of the model group was significantly slower than that of the control group. In the model group, the pyloric muscle was obviously hypertrophic. The expression of NGF mRNA and protein was significantly decreased in the model group, and the mRNA and protein expression of TrkA and p75 NTR in the model group were significantly decreased(P<0.05). Conclusion The decrease of TrkA, p75 NTR and NGF expression in pyloric tissue may be related to the pathogenesis of CHPS.

关 键 词：先天性肥厚性幽门狭窄 神经生长因子 TRKA P75NTR 

分 类 号：R573[医药卫生—消化系统]