沉默高表达CDX2胃癌细胞中HNF4α表达水平的变化  

Expression Changes of HNF4αafter Silenced High Expression CDX2 of Gastric Cancer Cells

作　　者：沈和德 杨文秀 褚明亮[3] 易韦[3] 张著学[3] SHEN Hede;YANG Wenxiu;CHU Mingliang;YI Wei;ZHANG Zhuxue(School of Basic Medicine,Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Pathology,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Pathology,the Affiliated People's Hospital of Guizhou Medical University,Guiyang 550002,Guizhou,China)

机构地区：[1]贵州医科大学基础医学院,贵州贵阳550004 [2]贵州医科大学附属医院病理科,贵州贵阳550004 [3]贵州医科大学附属人民医院病理科,贵州贵阳550002

出　　处：《贵州医科大学学报》2020年第8期899-903,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金地区基金项目(81560088);贵州省科技厅科研项目[黔科合J字(2015)2086];贵州省科技厅基金[LH字(2015)7108]。

摘　　要：目的:探讨沉默高表达尾核同源盒转录因子2(CDX2)胃癌细胞中后对肝细胞核因子4α(HNF4α)表达水平的变化。方法:采用Western blot检测CDX2和HNF4α两种蛋白在AGS、BGC-823、MGC-803、MKN-45和SGC-7901五种胃癌细胞的表达,筛选出2者在同一种细胞蛋白中均高表达的胃癌细胞;选择高表达CDX2和HNF4α的AGS细胞,采用Western lot检测沉默CDX2表达后细胞中HNF4α蛋白表达情况。结果:Western blot结果显示,CDX2与HNF4a蛋白在AGS胃癌细胞均有较高表达;与阴性对照相比,经沉默CDX2表达后的AGS胃癌细胞中CDX2蛋白为低表达,HNF4α蛋白也为低表达,差异有统计学意义(P<0.05)。结论:在高表达CDX2的AGS胃癌细胞中沉默CDX2后,CDX2与HNF4α蛋白均呈低表达水平。Objective:To investigate the expression level changes of HNF4αafter silenced high expression CDX2 of gastric cancer cells.Methods:Western blot was adopted to detect the expression of CDX2 and HNF4αproteins in five gastric cancer cells(AGS,BGC-823,MGC-803,MKN-45,and SGC-7901)and screen the gastric cancer cells with high expression of both CDX2 and HNF4αin the same one.Selecting high expression CDX2 and HNF4αAGS cells,then Western blot was used to detect HNF4αprotein expression after silenced expression of CDX2.Results:Western blot showed that CDX2 and HNF4 a proteins are highly expressed in AGS gastric cancer cells.Compared with the negative control,the expression of CDX2 in AGS gastric cancer cells was low after CDX2 gene silencing,and the expression of HNF4αprotein was also low,differences were statistically significant(P<0.05).Conclusion:After silencing CDX2 in high expression CDX2 of AGS gastric cancer cell,CDX2 and HNF4αprotein show low expression level.

关 键 词：胃肿瘤 基因沉默 尾核同源盒转录因子2蛋白 肝细胞核因子4α蛋白 蛋白免疫印迹 

分 类 号：R735.2[医药卫生—肿瘤]